Detailed information of TA system TA113223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 8850..9428
• Replicon: plasmid p4_010062
• Accession: NZ_CP033124
• Organism: Acinetobacter wuhouensis strain WCHAW010062

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CDG68_RS00710
• Protein ID: WP_087554407.1
• Coordinates: 8850..9182 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CDG68_RS00715
• Protein ID: WP_087554406.1
• Coordinates: 9183..9428 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG68_RS00685	5110..5820	-	711	WP_087554412.1	hypothetical protein	-
CDG68_RS00690	5991..6389	-	399	WP_087554411.1	hypothetical protein	-
CDG68_RS00695	6415..6861	-	447	WP_162994882.1	hypothetical protein	-
CDG68_RS00700	7114..7794	+	681	WP_087554409.1	recombinase family protein	-
CDG68_RS00705	7838..8737	-	900	WP_087554408.1	hypothetical protein	-
CDG68_RS00710	8850..9182	-	333	WP_087554407.1	endoribonuclease MazF	Toxin
CDG68_RS00715	9183..9428	-	246	WP_087554406.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDG68_RS22390	9516..9671	+	156	WP_162994883.1	hypothetical protein	-
CDG68_RS00720	9927..11462	-	1536	WP_087554405.1	FRG domain-containing protein	-
CDG68_RS00725	11734..12264	-	531	WP_087554404.1	plasmid replication DNA-binding protein	-
CDG68_RS00730	12343..13275	-	933	WP_087554403.1	replication initiation protein RepM	-
CDG68_RS22395	14137..14403	+	267	WP_153575742.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..15012	15012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-108)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12123.07 Da
• Isoelectric Point: 9.8488

>T113223 WP_087554407.1 NZ_CP033124:c9182-8850 [Acinetobacter wuhouensis]
MNQQYVPDAGDIIWINFTPQAGHEQAGHRPALVLSPASYNKLTSLLICCPLTRQVKGYPFEVNIAGNPTNVALADQVKSL
DWVARQAIKKGQATNKELAEVKKKINLLLK

Nucleotide

• Length: 333 bp

>T113223 NZ_CP033124:c9182-8850 [Acinetobacter wuhouensis]
ATGAATCAACAGTATGTACCTGACGCTGGTGACATTATTTGGATTAATTTCACACCTCAAGCAGGACACGAGCAAGCTGG
TCATAGACCAGCTTTAGTTTTAAGTCCTGCCTCATACAATAAATTAACAAGCTTATTGATTTGTTGCCCACTTACGCGAC
AAGTCAAAGGTTATCCATTTGAAGTCAATATTGCAGGAAATCCAACTAATGTTGCTTTGGCTGATCAAGTTAAAAGTCTG
GATTGGGTAGCCCGACAAGCAATTAAAAAAGGACAAGCAACGAACAAAGAACTAGCTGAGGTTAAAAAGAAAATTAACCT
ATTACTCAAATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8905.41 Da
• Isoelectric Point: 8.6015

>AT113223 WP_087554406.1 NZ_CP033124:c9428-9183 [Acinetobacter wuhouensis]
MELTIKRWGNSAALRLPADLLNGLNLKPESVVDVSMRNKAIIIKPIQKKSELSLEKLLAGITAENLHGEVDTGYAVGKEF
F

Nucleotide

• Length: 246 bp

>AT113223 NZ_CP033124:c9428-9183 [Acinetobacter wuhouensis]
ATGGAATTAACAATCAAGCGATGGGGTAATAGTGCGGCCTTACGGTTACCTGCTGATTTACTGAATGGCCTTAATCTAAA
GCCTGAAAGTGTTGTTGATGTATCCATGAGAAATAAGGCTATTATTATTAAACCAATTCAAAAAAAATCTGAACTTTCTT
TAGAAAAGTTATTAGCAGGCATAACTGCTGAAAACTTGCATGGCGAAGTAGATACTGGATATGCTGTAGGTAAGGAATTT
TTTTAA