Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 8436..9020 Replicon plasmid p3_010062
Accession NZ_CP033123
Organism Acinetobacter wuhouensis strain WCHAW010062

Toxin (Protein)


Gene name PumA Uniprot ID A0A7H2PX46
Locus tag CDG68_RS00615 Protein ID WP_049066985.1
Coordinates 8718..9020 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7H2PX47
Locus tag CDG68_RS00610 Protein ID WP_049066984.1
Coordinates 8436..8717 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDG68_RS00570 3829..4029 - 201 Protein_4 GABA permease -
CDG68_RS00575 3954..4382 - 429 Protein_5 hypothetical protein -
CDG68_RS00580 4552..4776 - 225 WP_004733276.1 DUF2798 domain-containing protein -
CDG68_RS00585 4790..5122 - 333 WP_004282109.1 hypothetical protein -
CDG68_RS00590 5366..5977 + 612 WP_004740252.1 recombinase family protein -
CDG68_RS00595 6905..7366 - 462 WP_000761345.1 DIP1984 family protein -
CDG68_RS00605 7903..8238 - 336 WP_004740263.1 carboxymuconolactone decarboxylase family protein -
CDG68_RS00610 8436..8717 - 282 WP_049066984.1 putative addiction module antidote protein Antitoxin
CDG68_RS00615 8718..9020 - 303 WP_049066985.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CDG68_RS00620 9189..9593 + 405 WP_087554372.1 hypothetical protein -
CDG68_RS00625 9632..10383 + 752 WP_087554373.1 IS5 family transposase -
CDG68_RS00630 10419..11018 + 600 WP_087554374.1 TetR/AcrR family transcriptional regulator -
CDG68_RS00635 11054..11458 + 405 WP_004762126.1 hypothetical protein -
CDG68_RS00645 11748..12254 - 507 WP_087554376.1 plasmid replication DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..16135 16135


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-91)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11589.07 Da        Isoelectric Point: 4.6838

>T113222 WP_049066985.1 NZ_CP033123:c9020-8718 [Acinetobacter wuhouensis]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLSGGDKS
TQSKDIKLALQLAQDLEEEL

Download         Length: 303 bp

>T113222 NZ_CP033123:c9020-8718 [Acinetobacter wuhouensis]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAACCAGTTGGTGAAGGTGTCTCTGAATTACGTTTCT
TCTTTGGACCAGGCTATAGGATTTACTACTGCAAACAAGGGCAAAGGGTTGTTATTCTTTTATCAGGTGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10151.75 Da        Isoelectric Point: 8.0018

>AT113222 WP_049066984.1 NZ_CP033123:c8717-8436 [Acinetobacter wuhouensis]
MAIKLRKWDSAEHLKTEEDMQAYLQVCIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Download         Length: 282 bp

>AT113222 NZ_CP033123:c8717-8436 [Acinetobacter wuhouensis]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAGGCTTACTTACAAGTATG
TATTGAAGAATCTAATGGTGATGCCGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACGGTG
ATTAAAGTTGTAAAAGCACTGAATTTGCGCTTAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H2PX46

Antitoxin

Source ID Structure
AlphaFold DB A0A7H2PX47

References