Detailed information of TA system TA113216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2200666..2201180
• Replicon: chromosome
• Accession: NZ_CP033114
• Organism: Staphylococcus aureus strain ST20130945

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: SAST45_RS10830
• Protein ID: WP_155682278.1
• Coordinates: 2200666..2200875 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: SAST45_RS10835
• Protein ID: WP_000138934.1
• Coordinates: 2200887..2201180 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAST45_RS10810	2196555..2197625	-	1071	WP_000159639.1	D-alanine--D-alanine ligase	-
SAST45_RS10815	2197943..2199145	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
SAST45_RS10820	2199306..2199989	-	684	Protein_2086	transposase	-
SAST45_RS10825	2200401..2200538	-	138	WP_000828354.1	hypothetical protein	-
SAST45_RS10830	2200666..2200875	-	210	WP_155682278.1	heavy-metal-associated domain-containing protein	Toxin
SAST45_RS10835	2200887..2201180	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SAST45_RS10840	2201346..2202830	+	1485	WP_000571560.1	cardiolipin synthase	-
SAST45_RS10845	2202858..2203505	+	648	WP_001187612.1	HD domain-containing protein	-
SAST45_RS14140	2203662..2203961	+	300	WP_001793952.1	hypothetical protein	-
SAST45_RS10855	2204118..2204990	-	873	WP_000725802.1	membrane protein insertase YidC	-
SAST45_RS10860	2205076..2205717	-	642	WP_000483150.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7796.80 Da
• Isoelectric Point: 4.1008

>T113216 WP_155682278.1 NZ_CP033114:c2200875-2200666 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVIINIIDGQVTVAYEAPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T113216 NZ_CP033114:c2200875-2200666 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGATTATTAATATAATAGACGGTCAGGTAACTGTAGCGTATGAGGCACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT113216 WP_000138934.1 NZ_CP033114:c2201180-2200887 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT113216 NZ_CP033114:c2201180-2200887 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0D1GBN9