Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2221768..2222297 Replicon chromosome
Accession NZ_CP033112
Organism Staphylococcus aureus strain ST20130944

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag SAST44_RS11065 Protein ID WP_000621176.1
Coordinates 2221768..2222130 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag SAST44_RS11070 Protein ID WP_000948331.1
Coordinates 2222127..2222297 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAST44_RS11045 2218747..2219517 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
SAST44_RS11050 2219492..2219971 - 480 WP_001190825.1 anti-sigma B factor RsbW -
SAST44_RS11055 2219973..2220299 - 327 WP_001052491.1 anti-sigma factor antagonist -
SAST44_RS11060 2220418..2221419 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
SAST44_RS11065 2221768..2222130 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SAST44_RS11070 2222127..2222297 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
SAST44_RS11075 2222382..2223530 - 1149 WP_001281140.1 alanine racemase -
SAST44_RS11080 2223596..2223955 - 360 WP_000581197.1 holo-ACP synthase -
SAST44_RS11085 2223959..2224450 - 492 WP_001286801.1 PH domain-containing protein -
SAST44_RS11090 2224443..2226020 - 1578 WP_001294646.1 PH domain-containing protein -
SAST44_RS11095 2226013..2226492 - 480 WP_001287082.1 hypothetical protein -
SAST44_RS11100 2226701..2227261 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T113199 WP_000621176.1 NZ_CP033112:c2222130-2221768 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T113199 NZ_CP033112:c2222130-2221768 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT113199 WP_000948331.1 NZ_CP033112:c2222297-2222127 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT113199 NZ_CP033112:c2222297-2222127 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References