Detailed information of TA system TA113175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1795671..1796322
• Replicon: chromosome
• Accession: NZ_CP033106
• Organism: Pantoea sp. 201603H

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EAE31_RS08035
• Protein ID: WP_154324864.1
• Coordinates: 1796140..1796322 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EAE31_RS08030
• Protein ID: WP_154324863.1
• Coordinates: 1795671..1796093 (-)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAE31_RS07990	1790735..1791394	-	660	WP_154324858.1	exodeoxyribonuclease X	-
EAE31_RS07995	1791519..1791749	-	231	WP_128174812.1	DNA polymerase III subunit theta	-
EAE31_RS08000	1791983..1792354	+	372	WP_154324859.1	CopC domain-containing protein YobA	-
EAE31_RS08005	1792358..1793236	+	879	WP_154324860.1	copper homeostasis membrane protein CopD	-
EAE31_RS08010	1793375..1793716	+	342	WP_128174806.1	YebY family protein	-
EAE31_RS08015	1794206..1794475	-	270	WP_154324861.1	tyrosine-type recombinase/integrase	-
EAE31_RS08020	1794532..1794936	+	405	WP_154325567.1	antitermination protein	-
EAE31_RS08025	1794970..1795122	-	153	WP_154324862.1	transmembrane anchored protein	-
EAE31_RS08030	1795671..1796093	-	423	WP_154324863.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EAE31_RS08035	1796140..1796322	-	183	WP_154324864.1	type II toxin-antitoxin system HicA family toxin	Toxin
EAE31_RS08040	1796556..1796765	+	210	Protein_1564	hypothetical protein	-
EAE31_RS08045	1796839..1797219	+	381	WP_154324865.1	hypothetical protein	-
EAE31_RS08050	1797542..1797985	+	444	WP_154324866.1	hypothetical protein	-
EAE31_RS08055	1798337..1798711	+	375	WP_154324867.1	QacE	-
EAE31_RS08060	1799153..1800100	+	948	WP_128174803.1	VirK/YbjX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1788670..1806541	17871
	-	flank	IS/Tn	-	-	1800576..1801607	1031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-133)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6945.11 Da
• Isoelectric Point: 10.7877

>T113175 WP_154324864.1 NZ_CP033106:c1796322-1796140 [Pantoea sp. 201603H]
MKSSELIKLLEKNGWELIRINGSHHHFSNPDFSNIITVPHPRKDLKTGTLQRILKDAKLK

Nucleotide

• Length: 183 bp

>T113175 NZ_CP033106:c1796322-1796140 [Pantoea sp. 201603H]
TTGAAAAGTTCGGAACTGATAAAACTGCTAGAGAAAAATGGATGGGAGTTGATACGGATTAACGGTAGTCACCACCATTT
TAGTAACCCGGATTTCTCTAACATAATCACAGTCCCACATCCTCGAAAAGACCTGAAAACAGGAACGCTTCAGAGAATTT
TAAAGGATGCGAAACTAAAATAA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15445.14 Da
• Isoelectric Point: 4.5713

>AT113175 WP_154324863.1 NZ_CP033106:c1796093-1795671 [Pantoea sp. 201603H]
MLYYAYVHVDKDGSSSGYFPDVTGCYFAGDTPDETIKDAHSAINAHFELMAEKGMSIPESSGQWGLDMSAESGSGEEDSK
GVWVAVDIDITKYLGKSERINITMPHLLIERIDSEVGKNSRYSSRSHFLAEAARKELSQL

Nucleotide

• Length: 423 bp

>AT113175 NZ_CP033106:c1796093-1795671 [Pantoea sp. 201603H]
ATGTTGTATTACGCTTACGTACATGTTGATAAAGATGGTTCATCAAGTGGTTACTTTCCCGATGTAACCGGGTGCTATTT
TGCTGGCGACACACCCGACGAGACCATAAAAGATGCCCATTCCGCGATCAATGCTCACTTTGAGTTAATGGCTGAAAAAG
GGATGAGCATTCCCGAGTCGTCAGGGCAATGGGGTCTGGATATGTCCGCAGAGAGTGGGAGCGGTGAAGAAGACTCTAAA
GGTGTATGGGTTGCCGTGGATATTGATATAACAAAATATCTCGGTAAATCTGAGCGCATCAATATCACAATGCCACACTT
GCTTATTGAGAGAATCGATAGCGAAGTTGGCAAAAATTCCCGGTATTCAAGCCGTAGCCATTTTTTGGCAGAGGCAGCGC
GGAAAGAACTTTCTCAGCTTTAA