Detailed information of TA system TA113164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5289984..5290621
• Replicon: chromosome
• Accession: NZ_CP033105
• Organism: Pseudomonas sp. LTJR-52

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EAW52_RS24450
• Protein ID: WP_122044446.1
• Coordinates: 5289984..5290394 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A3G2NF16
• Locus tag: EAW52_RS24455
• Protein ID: WP_074830119.1
• Coordinates: 5290391..5290621 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAW52_RS24425	5285527..5286366	+	840	WP_122044441.1	oxidoreductase	-
EAW52_RS24430	5286423..5287331	+	909	WP_122044442.1	AraC family transcriptional regulator	-
EAW52_RS24435	5287850..5288347	-	498	WP_122044443.1	hypothetical protein	-
EAW52_RS24440	5288445..5288861	-	417	WP_122044444.1	IS200/IS605 family transposase	-
EAW52_RS24445	5288883..5289938	+	1056	WP_122044445.1	transposase	-
EAW52_RS24450	5289984..5290394	-	411	WP_122044446.1	type II toxin-antitoxin system VapC family toxin	Toxin
EAW52_RS24455	5290391..5290621	-	231	WP_074830119.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EAW52_RS24460	5291147..5291638	+	492	WP_122044447.1	GNAT family N-acetyltransferase	-
EAW52_RS24465	5291840..5292763	-	924	WP_122044448.1	LysR family transcriptional regulator	-
EAW52_RS24470	5292874..5293626	+	753	WP_122044449.1	3-oxoacyl-ACP reductase FabG	-
EAW52_RS24475	5293639..5294235	+	597	WP_122044450.1	NADPH-dependent F420 reductase	-
EAW52_RS24480	5294330..5295607	-	1278	WP_122044451.1	translesion error-prone DNA polymerase V subunit UmuC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5163522..5424040	260518

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15168.64 Da
• Isoelectric Point: 7.2140

>T113164 WP_122044446.1 NZ_CP033105:c5290394-5289984 [Pseudomonas sp. LTJR-52]
VITYMLDTCICSFIMREHPASVIQRLATEVEQGNRFVISAITYAEMRYGQIGKKASTKHKTLVDEFVKRLDAVLPWDQRA
VDATVELMRVLTKAGMPIGPNDTAIAGHAVAIGCVLVTNNVREFSRVPSLVYEDWV

Nucleotide

• Length: 411 bp

>T113164 NZ_CP033105:c5290394-5289984 [Pseudomonas sp. LTJR-52]
GTGATCACCTATATGCTTGATACCTGCATCTGCTCTTTCATTATGCGAGAACATCCGGCCTCGGTGATCCAGCGTCTGGC
CACCGAAGTGGAGCAGGGCAACCGGTTTGTGATCTCGGCCATTACCTACGCCGAAATGCGTTATGGGCAGATCGGCAAAA
AGGCATCGACCAAGCACAAGACGCTGGTTGATGAGTTTGTAAAGCGCCTGGATGCCGTACTGCCTTGGGATCAGAGGGCA
GTCGATGCGACTGTCGAACTCATGCGCGTGCTAACCAAGGCCGGCATGCCAATTGGCCCTAACGATACCGCTATTGCAGG
GCATGCCGTGGCTATTGGCTGTGTACTTGTGACCAATAATGTGCGTGAGTTTAGCCGTGTCCCTAGCCTTGTTTACGAGG
ACTGGGTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8519.62 Da
• Isoelectric Point: 4.7405

>AT113164 WP_074830119.1 NZ_CP033105:c5290621-5290391 [Pseudomonas sp. LTJR-52]
MRTVSIFKNGKNQAVRLPNDMAYEGVGELEITREGDVITLRPARPSWASFAELPKADADFLQERQPVVGDEGRFNL

Nucleotide

• Length: 231 bp

>AT113164 NZ_CP033105:c5290621-5290391 [Pseudomonas sp. LTJR-52]
ATGCGTACCGTCTCTATTTTCAAAAATGGTAAGAATCAGGCCGTTCGCTTGCCCAATGACATGGCCTATGAAGGCGTCGG
GGAGCTTGAGATTACGCGGGAAGGTGATGTGATAACCCTGCGTCCTGCTCGGCCATCCTGGGCATCTTTTGCTGAGCTCC
CCAAGGCTGATGCTGACTTCCTGCAAGAGCGGCAACCAGTAGTCGGCGATGAAGGCAGATTCAATCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G2NF16