Detailed information of TA system TA113163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4924546..4925186
• Replicon: chromosome
• Accession: NZ_CP033105
• Organism: Pseudomonas sp. LTJR-52

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EAW52_RS22745
• Protein ID: WP_122044178.1
• Coordinates: 4924546..4924956 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EAW52_RS22750
• Protein ID: WP_122044179.1
• Coordinates: 4924956..4925186 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAW52_RS22725	4919715..4920719	-	1005	WP_122044175.1	NAD(P)-dependent oxidoreductase	-
EAW52_RS22730	4920920..4921444	+	525	WP_177411938.1	TetR/AcrR family transcriptional regulator	-
EAW52_RS22735	4921688..4923133	+	1446	WP_122044176.1	DnaJ domain-containing protein	-
EAW52_RS22740	4923352..4924386	-	1035	WP_122044177.1	GlxA family transcriptional regulator	-
EAW52_RS22745	4924546..4924956	-	411	WP_122044178.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EAW52_RS22750	4924956..4925186	-	231	WP_122044179.1	antitoxin	Antitoxin
EAW52_RS22755	4925677..4926249	+	573	WP_122044180.1	cupin domain-containing protein	-
EAW52_RS22760	4926377..4926850	-	474	WP_122044181.1	hypothetical protein	-
EAW52_RS22765	4926853..4927635	-	783	WP_122044182.1	hypothetical protein	-
EAW52_RS22775	4928195..4929436	+	1242	WP_162997692.1	hypothetical protein	-
EAW52_RS26055	4929647..4929829	-	183	WP_162997693.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4913207..4929436	16229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15429.66 Da
• Isoelectric Point: 7.3235

>T113163 WP_122044178.1 NZ_CP033105:c4924956-4924546 [Pseudomonas sp. LTJR-52]
MLKFMLDTNICIFTIKNRPQEVREAFKRHHGQMCISTVTLMELIYGAEKSSNTERNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVASPE

Nucleotide

• Length: 411 bp

>T113163 NZ_CP033105:c4924956-4924546 [Pseudomonas sp. LTJR-52]
ATGCTCAAGTTCATGCTCGACACCAATATTTGCATATTTACTATCAAGAACAGGCCCCAGGAGGTTCGAGAAGCCTTCAA
ACGGCACCACGGTCAAATGTGCATCAGCACTGTAACGCTAATGGAGTTGATCTATGGAGCGGAGAAGTCTTCTAACACTG
AGCGCAATCTGGCGGACGTAGAAGGCTTTGCGGCTCGCCTGGAGGTGCTCAAGTATGATCAGGAGGCTGCTGCCCACACT
GGACAACTTCGTGCTGAGCTTGCCCGCCTGGGTAAGCAGATTGGTCCCTATGACCAAATGATTGCCGGGCATGCACGCTC
CCAGGGACTGATTGTCGTTACCAATAACCGTCGAGAGTTCGACCGCGTACCTGGCTTGAGAGTTGAGGATTGGGTTGCCT
CACCCGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8478.43 Da
• Isoelectric Point: 4.2821

>AT113163 WP_122044179.1 NZ_CP033105:c4925186-4924956 [Pseudomonas sp. LTJR-52]
VEQGAVFQSNRSQAVRLPKAVALPDDVKRVDIVALGRARLITPAGESWDSWFDCEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT113163 NZ_CP033105:c4925186-4924956 [Pseudomonas sp. LTJR-52]
GTGGAACAAGGCGCAGTTTTTCAGAGCAATCGCAGTCAGGCCGTAAGGCTACCTAAGGCTGTTGCCCTACCAGATGACGT
AAAGCGCGTCGATATTGTTGCCCTTGGTCGGGCTCGACTTATCACTCCCGCAGGGGAGTCCTGGGATAGCTGGTTCGATT
GTGAGGGGGTTACTGCAGACTTCATGGCGGAGCGTGAGCAGCCGGCAGATCAGGAGCGCGAAACGTTCTGA