Detailed information of TA system TA113162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4532986..4534028
• Replicon: chromosome
• Accession: NZ_CP033104
• Organism: Pseudomonas sp. LTGT-11-2Z

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: D8767_RS21290
• Protein ID: WP_003153636.1
• Coordinates: 4532986..4533561 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: D8767_RS21295
• Protein ID: WP_003050245.1
• Coordinates: 4533558..4534028 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8767_RS21265	4529424..4530149	-	726	WP_122048403.1	CBASS effector endonuclease NucC	-
D8767_RS21270	4530188..4531090	-	903	WP_003153640.1	nucleotidyltransferase domain-containing protein	-
D8767_RS21275	4531090..4531590	-	501	WP_003090159.1	hypothetical protein	-
D8767_RS21280	4531587..4532057	-	471	WP_003153638.1	hypothetical protein	-
D8767_RS21285	4532054..4532968	-	915	WP_003050256.1	AAA family ATPase	-
D8767_RS21290	4532986..4533561	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
D8767_RS21295	4533558..4534028	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
D8767_RS21300	4534232..4534615	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
D8767_RS21305	4534612..4534845	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
D8767_RS21310	4534862..4535221	+	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
D8767_RS21315	4535234..4535644	+	411	WP_003821108.1	TIGR03750 family conjugal transfer protein	-
D8767_RS21320	4535641..4536333	+	693	WP_012614071.1	TIGR03746 family integrating conjugative element protein	-
D8767_RS21325	4536330..4537241	+	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
D8767_RS21330	4537231..4538649	+	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4502452..4539070	36618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T113162 WP_003153636.1 NZ_CP033104:c4533561-4532986 [Pseudomonas sp. LTGT-11-2Z]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T113162 NZ_CP033104:c4533561-4532986 [Pseudomonas sp. LTGT-11-2Z]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT113162 WP_003050245.1 NZ_CP033104:c4534028-4533558 [Pseudomonas sp. LTGT-11-2Z]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT113162 NZ_CP033104:c4534028-4533558 [Pseudomonas sp. LTGT-11-2Z]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68