Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 2443897..2444540 Replicon chromosome
Accession NZ_CP033102
Organism Enterobacter hormaechei strain L51

Toxin (Protein)


Gene name vagD Uniprot ID A0A5P6KDG5
Locus tag D8768_RS11685 Protein ID WP_021312768.1
Coordinates 2444124..2444540 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5P6KGW8
Locus tag D8768_RS11680 Protein ID WP_021312767.1
Coordinates 2443897..2444127 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8768_RS11665 2440464..2441219 + 756 WP_121909736.1 M48 family metallopeptidase -
D8768_RS11670 2441415..2442017 - 603 Protein_2276 hypothetical protein -
D8768_RS11675 2442037..2443184 + 1148 Protein_2277 IS3 family transposase -
D8768_RS11680 2443897..2444127 + 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
D8768_RS11685 2444124..2444540 + 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin Toxin
D8768_RS11690 2444638..2445540 - 903 WP_058672492.1 helix-turn-helix transcriptional regulator -
D8768_RS11695 2445721..2446152 + 432 WP_047732374.1 nuclear transport factor 2 family protein -
D8768_RS11700 2446321..2447229 - 909 WP_058672493.1 LysR family transcriptional regulator -
D8768_RS11705 2447358..2448668 + 1311 WP_058672494.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island mcr-9 / aph(6)-Id / aph(3'')-Ib / blaTEM-1B - 2430057..2523726 93669


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15175.60 Da        Isoelectric Point: 7.2452

>T113153 WP_021312768.1 NZ_CP033102:2444124-2444540 [Enterobacter hormaechei]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Download         Length: 417 bp

>T113153 NZ_CP033102:2444124-2444540 [Enterobacter hormaechei]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8722.81 Da        Isoelectric Point: 4.4692

>AT113153 WP_021312767.1 NZ_CP033102:2443897-2444127 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT113153 NZ_CP033102:2443897-2444127 [Enterobacter hormaechei]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P6KDG5

Antitoxin

Source ID Structure
AlphaFold DB A0A5P6KGW8

References