Detailed information of TA system TA113142

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 96540..97259
• Replicon: chromosome
• Accession: NZ_CP033102
• Organism: Enterobacter hormaechei strain L51

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A144XIR2
• Locus tag: D8768_RS00490
• Protein ID: WP_023305176.1
• Coordinates: 96540..96860 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: D8768_RS00495
• Protein ID: WP_032660963.1
• Coordinates: 96894..97259 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8768_RS00455	92433..93221	+	789	WP_157506443.1	winged helix-turn-helix domain-containing protein	-
D8768_RS00460	93223..93702	+	480	WP_022646606.1	hypothetical protein	-
D8768_RS00465	93945..94826	+	882	WP_032622044.1	helix-turn-helix domain-containing protein	-
D8768_RS00470	94891..95190	-	300	WP_023299004.1	XRE family transcriptional regulator	-
D8768_RS00475	95314..95655	-	342	WP_045621019.1	SymE family type I addiction module toxin	-
D8768_RS00480	95783..95944	+	162	WP_072050122.1	DUF3950 domain-containing protein	-
D8768_RS00485	96057..96263	-	207	WP_071788007.1	hypothetical protein	-
D8768_RS00490	96540..96860	-	321	WP_023305176.1	TA system toxin CbtA family protein	Toxin
D8768_RS00495	96894..97259	-	366	WP_032660963.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
D8768_RS00500	97291..97512	-	222	WP_023305178.1	DUF987 family protein	-
D8768_RS00505	97521..97991	-	471	WP_023305179.1	DNA repair protein RadC	-
D8768_RS00510	98061..98882	-	822	WP_023305180.1	DUF945 domain-containing protein	-
D8768_RS00515	99325..99516	-	192	WP_023305181.1	hypothetical protein	-
D8768_RS00520	99575..100414	-	840	WP_050582604.1	hypothetical protein	-
D8768_RS00525	100527..100961	-	435	WP_023305183.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	80410..116564	36154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12190.77 Da
• Isoelectric Point: 5.9559

>T113142 WP_023305176.1 NZ_CP033102:c96860-96540 [Enterobacter hormaechei]
MHISTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVHRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T113142 NZ_CP033102:c96860-96540 [Enterobacter hormaechei]
ATGCATATTTCAACTGTACCGGCAACGGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGATACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCTGGAGTGAATCTTACTGATGCTGTTAATTTCCTTGTGGAACGATTCGAGCTTGTGCGCATTGACCAGAAGGGTTTC
TCCTGGCAGGATCAAGAACCTTGGATAACATCTTTGGATGTTCATCGGGCACAGTTCAACCTTGGCTTGAAACGCTCATA
A

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13456.20 Da
• Isoelectric Point: 6.8421

>AT113142 WP_032660963.1 NZ_CP033102:c97259-96894 [Enterobacter hormaechei]
MQSTTLSGTSAENASCLQWGLKRNITPCFGARLVQEGNRLYFLADRAGFNGSFTDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT113142 NZ_CP033102:c97259-96894 [Enterobacter hormaechei]
ATGCAGTCGACCACCTTATCCGGCACATCGGCGGAGAACGCATCCTGCCTTCAGTGGGGTTTAAAACGTAATATCACGCC
ATGCTTTGGCGCTCGCCTGGTACAGGAAGGCAACCGCCTATACTTTCTGGCTGACCGGGCAGGATTTAATGGCTCATTCA
CTGACGATGAAGCTTTGCGTCTGGACCAGGCATTTCCACTGATGATGAAACAGCTTGAGCGGATGCTGACGGCTGGCGAG
CTTGATCCTCGCAGCCAGCACTGCGTCACACGGCATCACAACGGACTTACTTGTGAAGCTGACACTCTCGGCTCTCATGG
TTACGTTTACATTGCCATATATCCCCAGTCAGCTCTAACCAGATAA

Structures

Toxin

• AlphaFold DB: A0A144XIR2