Detailed information of TA system TA113140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF-doc
• Location: 12251..12834
• Replicon: plasmid unnamed1
• Accession: NZ_CP033099
• Organism: Staphylococcus warneri strain SWO

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: EAH52_RS12180
• Protein ID: WP_095325077.1
• Coordinates: 12251..12589 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: EAH52_RS12185
• Protein ID: WP_114561500.1
• Coordinates: 12586..12834 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAH52_RS12160	7874..9880	-	2007	WP_122135129.1	hypothetical protein	-
EAH52_RS12170	11081..11182	-	102	WP_117726018.1	type I toxin-antitoxin system Fst family toxin	-
EAH52_RS12175	11386..12205	+	820	Protein_14	hypothetical protein	-
EAH52_RS12180	12251..12589	-	339	WP_095325077.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EAH52_RS12185	12586..12834	-	249	WP_114561500.1	AbrB family transcriptional regulator	Antitoxin
EAH52_RS12190	12976..13362	-	387	WP_095325078.1	hypothetical protein	-
EAH52_RS12195	13414..13951	-	538	Protein_18	DUF536 domain-containing protein	-
EAH52_RS12200	14240..14617	-	378	WP_122135130.1	hypothetical protein	-
EAH52_RS12205	15545..15922	+	378	WP_122135130.1	hypothetical protein	-
EAH52_RS12210	16210..16748	+	539	Protein_21	DUF536 domain-containing protein	-
EAH52_RS12215	16799..17032	+	234	WP_122135131.1	hypothetical protein	-
EAH52_RS12220	17326..17574	+	249	WP_114561500.1	AbrB family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53165	53165

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13106.11 Da
• Isoelectric Point: 8.8341

>T113140 WP_095325077.1 NZ_CP033099:c12589-12251 [Staphylococcus warneri]
MSIKQFDIYYIDLDPTRGREKQKIRPCLIVNNQMTIDGTNFVWALPITNREKRYPLDIEVKTKKGLVTGVIDTLQIRALD
LNEREHNYKDELQDNLKNVVLQAIQTYLKPSS

Nucleotide

• Length: 339 bp

>T113140 NZ_CP033099:c12589-12251 [Staphylococcus warneri]
ATGAGCATCAAACAGTTTGATATCTACTATATCGACCTAGACCCAACTAGAGGTCGTGAAAAACAAAAAATCAGACCATG
TCTAATCGTAAATAATCAAATGACAATAGATGGCACAAATTTTGTTTGGGCTTTACCAATTACAAATCGAGAAAAGCGTT
ATCCATTGGATATTGAAGTGAAAACCAAAAAAGGGTTAGTCACAGGCGTTATAGACACGTTGCAGATACGTGCATTAGAC
CTCAATGAGCGCGAGCATAATTATAAAGACGAATTACAAGACAACTTAAAAAACGTCGTTTTGCAAGCTATACAGACGTA
TCTAAAACCATCTTCATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9162.20 Da
• Isoelectric Point: 4.4944

>AT113140 WP_114561500.1 NZ_CP033099:c12834-12586 [Staphylococcus warneri]
MNKTSERIFKSGNSKAISLGKQTIQLTDFEVGDKVEVQQVDGGLFITKKEQSVEDEITNFFKNGGKYTEPEIDFGESVGR
EI

Nucleotide

• Length: 249 bp

>AT113140 NZ_CP033099:c12834-12586 [Staphylococcus warneri]
ATGAACAAAACATCTGAACGTATCTTCAAAAGTGGTAACAGCAAAGCAATTAGCTTGGGCAAACAAACAATTCAACTCAC
TGATTTTGAGGTAGGGGATAAAGTCGAAGTACAACAAGTAGATGGCGGTTTATTCATCACTAAAAAAGAACAATCGGTTG
AAGATGAAATCACAAACTTCTTCAAAAATGGTGGTAAATACACTGAACCCGAAATTGACTTTGGAGAGAGTGTAGGTCGT
GAAATATGA