Detailed information of TA system TA113131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 37644..38442
• Replicon: plasmid pCP53-113k
• Accession: NZ_CP033097
• Organism: Escherichia coli strain CP53

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: EAH50_RS24885
• Protein ID: WP_109544792.1
• Coordinates: 37921..38442 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: EAH50_RS24880
• Protein ID: WP_001351987.1
• Coordinates: 37644..37913 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAH50_RS24855	33074..34414	-	1341	WP_000137333.1	AAA family ATPase	-
EAH50_RS24860	34458..35198	-	741	WP_001717320.1	hypothetical protein	-
EAH50_RS24865	35481..36248	+	768	WP_000342417.1	hypothetical protein	-
EAH50_RS24870	36301..36654	-	354	WP_162992119.1	hypothetical protein	-
EAH50_RS24875	36660..37325	-	666	WP_122057092.1	AAA family ATPase	-
EAH50_RS24880	37644..37913	+	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
EAH50_RS24885	37921..38442	+	522	WP_109544792.1	GNAT family N-acetyltransferase	Toxin
EAH50_RS24895	38611..38862	-	252	WP_122057094.1	hypothetical protein	-
EAH50_RS24900	38864..39556	-	693	WP_072644910.1	hypothetical protein	-
EAH50_RS24905	39570..39893	-	324	WP_000064175.1	hypothetical protein	-
EAH50_RS24910	39968..40813	-	846	WP_162992120.1	receptor-recognizing protein	-
EAH50_RS24915	40823..42373	-	1551	Protein_41	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113610	113610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19450.21 Da
• Isoelectric Point: 8.2462

>T113131 WP_109544792.1 NZ_CP033097:37921-38442 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLDFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T113131 NZ_CP033097:37921-38442 [Escherichia coli]
GTGAGCAATACAACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGATATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGACTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT113131 WP_001351987.1 NZ_CP033097:37644-37913 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT113131 NZ_CP033097:37644-37913 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7