Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2388178..2388816 Replicon chromosome
Accession NZ_CP033096
Organism Escherichia coli strain CP53

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EAH50_RS13835 Protein ID WP_000813794.1
Coordinates 2388640..2388816 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EAH50_RS13830 Protein ID WP_001270286.1
Coordinates 2388178..2388594 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAH50_RS13810 2383330..2384271 - 942 WP_122056950.1 ABC transporter permease -
EAH50_RS13815 2384272..2385285 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
EAH50_RS13820 2385303..2386448 - 1146 WP_000047418.1 ABC transporter substrate-binding protein -
EAH50_RS13825 2386693..2388099 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
EAH50_RS13830 2388178..2388594 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EAH50_RS13835 2388640..2388816 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EAH50_RS13840 2389038..2389268 + 231 WP_000494244.1 YncJ family protein -
EAH50_RS13845 2389360..2391321 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EAH50_RS13850 2391394..2391930 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
EAH50_RS13855 2391983..2393197 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T113126 WP_000813794.1 NZ_CP033096:c2388816-2388640 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T113126 NZ_CP033096:c2388816-2388640 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT113126 WP_001270286.1 NZ_CP033096:c2388594-2388178 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT113126 NZ_CP033096:c2388594-2388178 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References