Detailed information of TA system TA113110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 63099..63646
• Replicon: plasmid pCP53-92k
• Accession: NZ_CP033095
• Organism: Escherichia coli strain CP53

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: EAH50_RS02005
• Protein ID: WP_050858704.1
• Coordinates: 63365..63646 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A829FT18
• Locus tag: EAH50_RS02000
• Protein ID: WP_000079938.1
• Coordinates: 63099..63368 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAH50_RS01970	59444..60658	-	1215	WP_001532073.1	type II site-specific deoxyribonuclease	-
EAH50_RS01975	60897..61355	+	459	Protein_67	recombinase family protein	-
EAH50_RS01980	61410..62114	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
EAH50_RS25385	62886..63026	+	141	WP_160784086.1	hypothetical protein	-
EAH50_RS02000	63099..63368	+	270	WP_000079938.1	hypothetical protein	Antitoxin
EAH50_RS02005	63365..63646	+	282	WP_050858704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EAH50_RS25390	63699..63836	+	138	WP_152931508.1	hypothetical protein	-
EAH50_RS02010	63982..65144	+	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
EAH50_RS02015	65174..65314	-	141	Protein_74	NADP-dependent phosphogluconate dehydrogenase	-
EAH50_RS02020	65507..66613	-	1107	WP_032277577.1	glycosyltransferase family 4 protein	-
EAH50_RS02025	66623..67246	-	624	WP_032277575.1	NeuD/PglB/VioB family sugar acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B) / aac(3)-IId	gnd / gmd / manB / manC / galF	1..92168	92168
	-	inside	IScluster/Tn	-	-	57611..65144	7533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10860.58 Da
• Isoelectric Point: 8.4615

>T113110 WP_050858704.1 NZ_CP033095:63365-63646 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMVAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSPWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T113110 NZ_CP033095:63365-63646 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGTCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTGCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCCGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10251.64 Da
• Isoelectric Point: 10.1734

>AT113110 WP_000079938.1 NZ_CP033095:63099-63368 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT113110 NZ_CP033095:63099-63368 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829FT18