Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 18105..18627 Replicon plasmid pCP53-38k
Accession NZ_CP033093
Organism Escherichia coli strain CP53

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag EAH50_RS00105 Protein ID WP_000220560.1
Coordinates 18105..18386 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag EAH50_RS00110 Protein ID WP_000121743.1
Coordinates 18376..18627 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAH50_RS00070 13373..14437 + 1065 WP_001269817.1 fimbrial protein -
EAH50_RS00075 14480..14680 - 201 WP_000350095.1 cell surface composition regulator GlgS -
EAH50_RS00080 14949..15542 + 594 WP_000599943.1 DUF1449 family protein -
EAH50_RS00085 15576..16273 + 698 Protein_16 IS1 family transposase -
EAH50_RS00090 16337..16648 + 312 WP_001452736.1 hypothetical protein -
EAH50_RS00095 16857..17339 + 483 WP_077874353.1 restriction endonuclease -
EAH50_RS00100 17333..18030 - 698 Protein_19 IS1-like element IS1A family transposase -
EAH50_RS00105 18105..18386 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EAH50_RS00110 18376..18627 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
EAH50_RS00125 19861..20697 + 837 WP_001050931.1 replication initiation protein -
EAH50_RS00130 20737..21183 + 447 Protein_23 hypothetical protein -
EAH50_RS00135 21251..21676 + 426 WP_000202942.1 hypothetical protein -
EAH50_RS00140 21673..22650 - 978 WP_024245156.1 hypothetical protein -
EAH50_RS00145 22675..22956 - 282 WP_001185482.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrS1 / blaTEM-1B / tet(M) - 1..38797 38797


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T113109 WP_000220560.1 NZ_CP033093:c18386-18105 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T113109 NZ_CP033093:c18386-18105 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT113109 WP_000121743.1 NZ_CP033093:c18627-18376 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT113109 NZ_CP033093:c18627-18376 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References