Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4384238..4384735 Replicon chromosome
Accession NZ_CP033092
Organism Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EAS44_RS22630 Protein ID WP_001173343.1
Coordinates 4384238..4384411 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag EAS44_RS22635 Protein ID WP_001312331.1
Coordinates 4384439..4384735 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAS44_RS22620 4380908..4382365 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
EAS44_RS22625 4382602..4384119 + 1518 WP_000003806.1 lysine--tRNA ligase -
EAS44_RS22630 4384238..4384411 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EAS44_RS22635 4384439..4384735 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EAS44_RS22640 4384963..4385235 - 273 WP_000405647.1 N-acetyltransferase -
EAS44_RS22645 4385247..4385477 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
EAS44_RS22650 4385658..4387289 + 1632 WP_001216482.1 sensor histidine kinase -
EAS44_RS22655 4387286..4388005 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - papX 4313342..4384735 71393


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T113107 WP_001173343.1 NZ_CP033092:c4384411-4384238 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T113107 NZ_CP033092:c4384411-4384238 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT113107 WP_001312331.1 NZ_CP033092:c4384735-4384439 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT113107 NZ_CP033092:c4384735-4384439 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References