Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4064884..4065460 Replicon chromosome
Accession NZ_CP033092
Organism Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag EAS44_RS20925 Protein ID WP_001299718.1
Coordinates 4065173..4065460 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0E2KXJ8
Locus tag EAS44_RS20920 Protein ID WP_000063159.1
Coordinates 4064884..4065186 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAS44_RS20905 4061473..4063623 + 2151 WP_001299714.1 pyruvate/proton symporter BtsT -
EAS44_RS20910 4063673..4063876 + 204 WP_000467859.1 YbdD/YjiX family protein -
EAS44_RS20915 4063887..4064843 + 957 WP_001299717.1 GTPase -
EAS44_RS20920 4064884..4065186 - 303 WP_000063159.1 BrnA antitoxin family protein Antitoxin
EAS44_RS20925 4065173..4065460 - 288 WP_001299718.1 BrnT family toxin Toxin
EAS44_RS20930 4065657..4066823 + 1167 Protein_3886 restriction endonuclease -
EAS44_RS20935 4066887..4068506 + 1620 WP_001029740.1 type I restriction-modification system subunit M -
EAS44_RS20940 4068496..4069887 + 1392 WP_000535334.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T113102 WP_001299718.1 NZ_CP033092:c4065460-4065173 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T113102 NZ_CP033092:c4065460-4065173 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11536.01 Da        Isoelectric Point: 9.4646

>AT113102 WP_000063159.1 NZ_CP033092:c4065186-4064884 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT113102 NZ_CP033092:c4065186-4064884 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAATTGAAGGCGCTGGCGAA
TAAATCCGACGAAGAGATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2KXJ8

References