Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 941203..941857 Replicon chromosome
Accession NZ_CP033092
Organism Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EAS44_RS05440 Protein ID WP_000244781.1
Coordinates 941450..941857 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EAS44_RS05435 Protein ID WP_000354046.1
Coordinates 941203..941469 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAS44_RS05415 937291..938724 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
EAS44_RS05420 938769..939080 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
EAS44_RS05425 939244..939903 + 660 WP_000250274.1 hemolysin III family protein -
EAS44_RS05430 939980..940960 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
EAS44_RS05435 941203..941469 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EAS44_RS05440 941450..941857 + 408 WP_000244781.1 protein YgfX Toxin
EAS44_RS05445 941897..942418 - 522 WP_001055874.1 flavodoxin FldB -
EAS44_RS05450 942530..943426 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
EAS44_RS05455 943451..944161 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EAS44_RS05460 944167..945900 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T113083 WP_000244781.1 NZ_CP033092:941450-941857 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T113083 NZ_CP033092:941450-941857 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT113083 WP_000354046.1 NZ_CP033092:941203-941469 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT113083 NZ_CP033092:941203-941469 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References