Detailed information of TA system TA113073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 43811..44336
• Replicon: plasmid unnamed
• Accession: NZ_CP033089
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain SEO

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: EAH54_RS00305
• Protein ID: WP_001159863.1
• Coordinates: 44031..44336 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: EAH54_RS00300
• Protein ID: WP_000813641.1
• Coordinates: 43811..44029 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAH54_RS00270	39425..39811	+	387	WP_000751876.1	YgiW/YdeI family stress tolerance OB fold protein	-
EAH54_RS00275	39864..40025	+	162	WP_015059604.1	hypothetical protein	-
EAH54_RS00280	40352..41341	-	990	WP_000461382.1	RepB family plasmid replication initiator protein	-
EAH54_RS00285	41835..42130	-	296	Protein_55	cytoplasmic protein	-
EAH54_RS23915	42142..42570	+	429	Protein_56	hypothetical protein	-
EAH54_RS00295	42614..43135	-	522	WP_077681952.1	hypothetical protein	-
EAH54_RS00300	43811..44029	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
EAH54_RS00305	44031..44336	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
EAH54_RS00310	44338..44628	+	291	WP_001266176.1	hypothetical protein	-
EAH54_RS00315	44625..45146	+	522	WP_000198608.1	hypothetical protein	-
EAH54_RS00320	45181..45963	+	783	WP_000082169.1	site-specific integrase	-
EAH54_RS00325	45972..46685	+	714	WP_000545756.1	EAL domain-containing protein	-
EAH54_RS00330	46689..47198	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
EAH54_RS00335	47192..47677	+	486	WP_000905606.1	membrane protein	-
EAH54_RS23780	47954..48241	-	288	WP_071530243.1	hypothetical protein	-
EAH54_RS00340	48397..48957	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59373	59373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T113073 WP_001159863.1 NZ_CP033089:44031-44336 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T113073 NZ_CP033089:44031-44336 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT113073 WP_000813641.1 NZ_CP033089:43811-44029 [Salmonella enterica subsp. enterica serovar Enteritidis]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT113073 NZ_CP033089:43811-44029 [Salmonella enterica subsp. enterica serovar Enteritidis]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6