Detailed information of TA system TA113068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5073135..5073798
• Replicon: chromosome
• Accession: NZ_CP033076
• Organism: Buttiauxella sp. 3AFRM03

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D8682_RS25250
• Protein ID: WP_121815475.1
• Coordinates: 5073610..5073798 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D8682_RS25245
• Protein ID: WP_121815474.1
• Coordinates: 5073135..5073545 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8682_RS25205	5068141..5068383	+	243	WP_121815466.1	hypothetical protein	-
D8682_RS25210	5068386..5068784	+	399	WP_121815467.1	hypothetical protein	-
D8682_RS25215	5069329..5069508	+	180	WP_121815468.1	hypothetical protein	-
D8682_RS25220	5069664..5069978	+	315	WP_121815469.1	DUF968 domain-containing protein	-
D8682_RS25225	5069975..5071456	+	1482	WP_121815470.1	DNA cytosine methyltransferase	-
D8682_RS25230	5071453..5071593	+	141	WP_121815471.1	YlcG family protein	-
D8682_RS25235	5071590..5072192	+	603	WP_121815472.1	hypothetical protein	-
D8682_RS25240	5072567..5072836	+	270	WP_162303051.1	hypothetical protein	-
D8682_RS25245	5073135..5073545	-	411	WP_121815474.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D8682_RS25250	5073610..5073798	-	189	WP_121815475.1	type II toxin-antitoxin system HicA family toxin	Toxin
D8682_RS25255	5073934..5074239	+	306	WP_162303052.1	hypothetical protein	-
D8682_RS25260	5074283..5074663	+	381	WP_121815477.1	hypothetical protein	-
D8682_RS25265	5074845..5075336	+	492	WP_121815478.1	DUF1441 family protein	-
D8682_RS25270	5075336..5077411	+	2076	WP_121815479.1	phage terminase large subunit family protein	-
D8682_RS25275	5077423..5077647	+	225	WP_121815480.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5052302..5118616	66314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-53)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6901.14 Da
• Isoelectric Point: 11.9739

>T113068 WP_121815475.1 NZ_CP033076:c5073798-5073610 [Buttiauxella sp. 3AFRM03]
MSSAELIKRLIADGWVKQRQSGSHVTLTKPGVRKIITVPHPRKDTSRGIIRQAQEISGLKLL

Nucleotide

• Length: 189 bp

>T113068 NZ_CP033076:c5073798-5073610 [Buttiauxella sp. 3AFRM03]
ATGAGTAGTGCAGAGTTAATAAAACGACTAATAGCTGATGGATGGGTTAAGCAGCGGCAAAGTGGGAGCCACGTAACGCT
GACTAAACCAGGGGTTCGGAAAATCATCACCGTACCCCACCCCAGAAAAGACACATCACGGGGGATTATCCGGCAAGCTC
AGGAGATATCAGGTCTTAAGCTGTTGTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15333.30 Da
• Isoelectric Point: 4.5904

>AT113068 WP_121815474.1 NZ_CP033076:c5073545-5073135 [Buttiauxella sp. 3AFRM03]
MIYPLFIFKTESGEYDGYFPDVEGCFFAGDTLEAAIRDAESAFSQHMEVLTEQGEHVPAPKEPGIYVGDERLTQDGGFMA
LLDIDPGKYETKAVKFNLTMPGNLITAIDRYIEKNGRYKNRSSFLAELARKEIARH

Nucleotide

• Length: 411 bp

>AT113068 NZ_CP033076:c5073545-5073135 [Buttiauxella sp. 3AFRM03]
ATGATTTATCCACTTTTTATATTCAAAACTGAAAGCGGCGAGTATGACGGTTATTTCCCTGATGTTGAGGGGTGTTTCTT
TGCCGGTGATACTCTGGAAGCGGCTATACGTGATGCTGAGTCAGCTTTCAGCCAGCACATGGAAGTATTAACCGAGCAAG
GCGAGCATGTGCCAGCACCTAAAGAGCCAGGCATTTATGTTGGAGATGAAAGGCTGACTCAGGATGGTGGCTTTATGGCG
CTGCTGGATATCGATCCAGGCAAGTATGAAACCAAGGCGGTAAAATTTAATTTAACGATGCCTGGCAATCTCATCACGGC
TATCGACCGATATATTGAAAAGAATGGTCGCTACAAAAACCGTTCTTCTTTCCTTGCCGAACTGGCGAGAAAAGAAATTG
CACGGCATTAA