Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1864390..1865012 Replicon chromosome
Accession NZ_CP033076
Organism Buttiauxella sp. 3AFRM03

Toxin (Protein)


Gene name Hha Uniprot ID A0A3G2IEF5
Locus tag D8682_RS10160 Protein ID WP_064549071.1
Coordinates 1864794..1865012 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3G2ICZ8
Locus tag D8682_RS10155 Protein ID WP_064549070.1
Coordinates 1864390..1864764 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8682_RS10145 1859463..1860662 + 1200 WP_064549066.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D8682_RS10150 1860685..1863834 + 3150 WP_121814204.1 multidrug efflux RND transporter permease subunit AcrB -
D8682_RS10155 1864390..1864764 + 375 WP_064549070.1 Hha toxicity modulator TomB Antitoxin
D8682_RS10160 1864794..1865012 + 219 WP_064549071.1 hemolysin expression modulator Hha Toxin
D8682_RS10165 1865208..1865774 + 567 WP_064549075.1 maltose O-acetyltransferase -
D8682_RS10170 1865877..1866335 + 459 WP_064549077.1 YlaC family protein -
D8682_RS10175 1866375..1866515 - 141 WP_034458060.1 type B 50S ribosomal protein L36 -
D8682_RS10180 1866810..1867847 + 1038 WP_064549079.1 isopenicillin N synthase family oxygenase -
D8682_RS10185 1867866..1868675 + 810 WP_064549081.1 MetQ/NlpA family ABC transporter substrate-binding protein -
D8682_RS10190 1868672..1869463 + 792 WP_121814205.1 ATP-binding cassette domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8611.01 Da        Isoelectric Point: 8.9108

>T113062 WP_064549071.1 NZ_CP033076:1864794-1865012 [Buttiauxella sp. 3AFRM03]
MTTKPLTKTDYLMRLRRCQTIETLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWTFIR

Download         Length: 219 bp

>T113062 NZ_CP033076:1864794-1865012 [Buttiauxella sp. 3AFRM03]
ATGACTACTAAACCATTAACTAAGACTGATTATTTGATGCGTCTGCGACGCTGCCAAACAATTGAGACCTTAGAACGTGT
AATTGAAAAAAATAAATACGAATTATCAGATAATGAATTGGCGGTATTTTATTCAGCTGCCGACCACCGACTTGCTGAAC
TCACCATGAATAAGCTTTACGATAAAATACCACCCTCTGTATGGACATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14521.28 Da        Isoelectric Point: 4.9132

>AT113062 WP_064549070.1 NZ_CP033076:1864390-1864764 [Buttiauxella sp. 3AFRM03]
MDEYSPKRHDIAQLKFLCENLYHDCLTNLGESNHGWVNDPTSAINLQLNELIEHIAAFALNYKIKYDDESKLIEQIDEYL
DDTFMLFSNYGINAHDLQKWRKSGNRLFRCFVTESRANPVSLSF

Download         Length: 375 bp

>AT113062 NZ_CP033076:1864390-1864764 [Buttiauxella sp. 3AFRM03]
ATGGACGAATACTCACCAAAACGGCATGATATTGCGCAGCTTAAATTTCTCTGCGAAAACCTGTATCATGACTGCCTTAC
CAACCTTGGCGAGAGTAATCATGGTTGGGTTAATGATCCTACTTCGGCCATTAACTTGCAGTTGAATGAATTGATTGAGC
ACATAGCTGCCTTCGCACTTAATTACAAAATTAAGTATGATGATGAGAGTAAATTGATTGAACAAATTGACGAATATTTG
GACGATACCTTTATGCTGTTCAGCAACTACGGCATCAATGCACACGATTTGCAGAAATGGCGTAAGTCCGGGAATCGATT
ATTCCGATGTTTCGTCACAGAGAGCCGCGCTAACCCTGTAAGCCTCTCCTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G2IEF5

Antitoxin

Source ID Structure
AlphaFold DB A0A3G2ICZ8

References