Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1755018..1755673 Replicon chromosome
Accession NZ_CP033076
Organism Buttiauxella sp. 3AFRM03

Toxin (Protein)


Gene name vapC Uniprot ID A0A3G2IDJ0
Locus tag D8682_RS09715 Protein ID WP_064548895.1
Coordinates 1755018..1755407 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3G2ICR3
Locus tag D8682_RS09720 Protein ID WP_064548897.1
Coordinates 1755410..1755673 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8682_RS09680 1750565..1751746 + 1182 WP_121814151.1 FMN-dependent L-lactate dehydrogenase LldD -
D8682_RS09685 1751790..1752092 - 303 WP_064548886.1 hypothetical protein -
D8682_RS09690 1752145..1752411 - 267 WP_121814152.1 type II toxin-antitoxin system RelE/ParE family toxin -
D8682_RS09695 1752627..1753055 - 429 WP_121814153.1 OsmC family protein -
D8682_RS09700 1753214..1753747 + 534 WP_064548891.1 tetratricopeptide repeat protein -
D8682_RS09705 1753754..1754275 + 522 WP_083963455.1 RNA polymerase sigma factor -
D8682_RS09710 1754272..1755021 + 750 WP_121814154.1 anti-sigma factor -
D8682_RS09715 1755018..1755407 - 390 WP_064548895.1 type II toxin-antitoxin system VapC family toxin Toxin
D8682_RS09720 1755410..1755673 - 264 WP_064548897.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
D8682_RS09725 1755933..1756214 - 282 WP_121814155.1 acetolactate synthase small subunit -
D8682_RS09730 1756218..1757906 - 1689 WP_121814156.1 acetolactate synthase large subunit -
D8682_RS09735 1758017..1758115 - 99 WP_074388597.1 ilvB operon leader peptide IvbL -
D8682_RS09740 1758244..1759266 - 1023 WP_121814157.1 GTP-binding protein -
D8682_RS09745 1759282..1759479 - 198 WP_034456870.1 YbdD/YjiX family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-120)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14403.59 Da        Isoelectric Point: 9.7107

>T113060 WP_064548895.1 NZ_CP033076:c1755407-1755018 [Buttiauxella sp. 3AFRM03]
MYMLDTNTVSYLFRQHPKVIEKLAQTRPVEVCISSVTEAELLYGVAKRQSKNLAAAVQGFLETVSVYAWDSEAAKSYGQL
RAAMEKKGKVLGHLDQLIAAHALSRNATVVTSDRAFTMVGRLRVEDWTR

Download         Length: 390 bp

>T113060 NZ_CP033076:c1755407-1755018 [Buttiauxella sp. 3AFRM03]
ATGTACATGCTGGATACCAACACGGTGAGCTATCTTTTCCGTCAGCATCCTAAAGTAATAGAAAAGCTGGCTCAAACGCG
CCCTGTAGAAGTGTGCATTTCTAGTGTAACGGAAGCTGAGCTGTTATATGGCGTGGCTAAGCGCCAGAGTAAAAATCTGG
CTGCGGCGGTGCAGGGTTTTCTGGAAACAGTATCGGTCTACGCGTGGGACAGCGAAGCGGCAAAAAGCTACGGTCAGTTA
CGAGCGGCGATGGAAAAGAAAGGCAAAGTATTGGGTCATCTTGACCAGTTAATTGCTGCTCACGCCCTGAGCCGAAACGC
GACGGTGGTCACCAGCGATCGTGCTTTTACCATGGTCGGGCGTTTACGCGTTGAGGACTGGACGCGCTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10009.36 Da        Isoelectric Point: 8.5606

>AT113060 WP_064548897.1 NZ_CP033076:c1755673-1755410 [Buttiauxella sp. 3AFRM03]
MERVAKLFKNGRNQAVRLPVEFEFDTDRVYIRKDKEGNVILSKNPAKPEGWGNVFALVKKAKVPDNFLDEQERNQSIADR
DPFAGMD

Download         Length: 264 bp

>AT113060 NZ_CP033076:c1755673-1755410 [Buttiauxella sp. 3AFRM03]
ATGGAACGCGTCGCGAAGCTTTTTAAAAACGGCAGAAACCAGGCGGTGAGGTTACCGGTGGAATTTGAGTTTGATACCGA
CAGGGTGTATATCCGCAAAGACAAGGAGGGAAACGTGATTCTTTCAAAAAATCCAGCCAAACCAGAAGGATGGGGTAACG
TTTTTGCTTTAGTGAAGAAAGCAAAAGTGCCTGATAACTTCCTTGATGAGCAGGAGCGTAACCAGAGTATTGCCGACAGA
GATCCCTTTGCAGGGATGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G2IDJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G2ICR3

References