Detailed information of TA system TA113049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 381005..381759
• Replicon: chromosome
• Accession: NZ_CP033076
• Organism: Buttiauxella sp. 3AFRM03

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: D8682_RS02960
• Protein ID: WP_121813491.1
• Coordinates: 381005..381505 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A3G2IA09
• Locus tag: D8682_RS02965
• Protein ID: WP_064546702.1
• Coordinates: 381496..381759 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8682_RS02930	376306..377361	-	1056	WP_121813490.1	LysR family transcriptional regulator	-
D8682_RS02935	377607..377996	+	390	WP_064546712.1	tautomerase family protein	-
D8682_RS02940	378132..378455	+	324	WP_064546710.1	hypothetical protein	-
D8682_RS02945	378494..378787	+	294	WP_064546708.1	DUF1330 domain-containing protein	-
D8682_RS02950	379248..379883	+	636	WP_121815621.1	hypothetical protein	-
D8682_RS02955	379927..380806	-	880	Protein_365	ISNCY family transposase	-
D8682_RS02960	381005..381505	-	501	WP_121813491.1	GNAT family N-acetyltransferase	Toxin
D8682_RS02965	381496..381759	-	264	WP_064546702.1	DUF1778 domain-containing protein	Antitoxin
D8682_RS02970	381958..382185	-	228	WP_064546700.1	hypothetical protein	-
D8682_RS02975	382298..383308	-	1011	WP_121813492.1	Ldh family oxidoreductase	-
D8682_RS02980	383329..384894	-	1566	WP_064546695.1	APC family permease	-
D8682_RS02985	384915..386531	-	1617	WP_121813493.1	aldehyde dehydrogenase (NADP(+))	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	379927..380499	572

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18267.10 Da
• Isoelectric Point: 9.7410

>T113049 WP_121813491.1 NZ_CP033076:c381505-381005 [Buttiauxella sp. 3AFRM03]
VGLVTAPEPLTSSHQLAEFVSGESVLDDWLKQRGLKNHAYGAARTFVICKKESKQVAGFYSLATGSVNHTEATGGLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVADNIGVRAIMVHALTEKAKQFYIHHGFKPLQTQERTLFFKLP
KDVEGT

Nucleotide

• Length: 501 bp

>T113049 NZ_CP033076:c381505-381005 [Buttiauxella sp. 3AFRM03]
GTGGGACTAGTAACAGCTCCTGAGCCTCTGACAAGTTCTCATCAGCTAGCGGAATTCGTGAGTGGAGAGTCAGTTCTTGA
TGATTGGTTAAAGCAAAGAGGGTTAAAAAACCACGCTTACGGTGCTGCAAGGACGTTTGTCATTTGTAAGAAGGAATCGA
AACAAGTAGCGGGCTTTTACTCACTGGCAACTGGAAGTGTCAACCATACCGAAGCTACGGGTGGTCTTCGTCGTAATATG
CCGGACCCAATACCCGTTATTATACTAGCGCGTCTGGCAGTGGACCTTTCTTTCCGGGGAAAAGGTCTGGGAGCCGATCT
GCTTCACGATGCAGTACTGCGATGCTACAGGGTGGCCGATAATATCGGTGTCCGGGCCATTATGGTCCATGCACTTACTG
AAAAAGCCAAACAATTCTATATTCATCATGGTTTTAAACCATTGCAAACGCAGGAGCGAACATTATTTTTTAAACTTCCG
AAAGATGTGGAGGGGACTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9990.42 Da
• Isoelectric Point: 4.6313

>AT113049 WP_064546702.1 NZ_CP033076:c381759-381496 [Buttiauxella sp. 3AFRM03]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPLADDPAVKKLL
ARKPQWD

Nucleotide

• Length: 264 bp

>AT113049 NZ_CP033076:c381759-381496 [Buttiauxella sp. 3AFRM03]
ATGAAATCAGATGTTCAACTCAACCTTAGAGCTAAGGAGTCCCAGCGGGCGCTCATTGATGCAGCCGCCGAGATCCTCCA
TAAATCGCGAACAGACTTTATATTGGAAATGGCCTGTCAGGCGGCAGAGAATGTGATACTTGATCGACGTGTATTCAATT
TTAATGACGAACAATACGCTGAGTTCATAGATATGCTTGATGCTCCGCTTGCAGATGACCCAGCCGTTAAGAAATTACTC
GCAAGGAAGCCTCAGTGGGACTAG

Structures

Antitoxin

• AlphaFold DB: A0A3G2IA09