Detailed information of TA system TA113048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 114028..114605
• Replicon: chromosome
• Accession: NZ_CP033076
• Organism: Buttiauxella sp. 3AFRM03

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D8682_RS01810
• Protein ID: WP_121813346.1
• Coordinates: 114273..114605 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A3G2I9E0
• Locus tag: D8682_RS01805
• Protein ID: WP_064547184.1
• Coordinates: 114028..114273 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8682_RS01785	110261..111205	+	945	WP_121815611.1	hypothetical protein	-
D8682_RS01790	111246..111533	+	288	WP_121813344.1	type VI secretion system PAAR protein	-
D8682_RS01795	111842..113404	+	1563	WP_121813345.1	4-hydroxyphenylacetate 3-monooxygenase, oxygenase component	-
D8682_RS01800	113424..113930	+	507	WP_064547185.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
D8682_RS01805	114028..114273	+	246	WP_064547184.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D8682_RS01810	114273..114605	+	333	WP_121813346.1	endoribonuclease MazF	Toxin
D8682_RS01815	114697..115647	-	951	WP_064547181.1	universal stress protein UspE	-
D8682_RS01820	115784..116536	-	753	WP_064547179.1	fumarate/nitrate reduction transcriptional regulator Fnr	-
D8682_RS01825	116737..117252	-	516	WP_064547177.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
D8682_RS01830	117265..118791	-	1527	WP_121813347.1	p-aminobenzoyl-glutamate transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-109)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11934.73 Da
• Isoelectric Point: 6.9247

>T113048 WP_121813346.1 NZ_CP033076:114273-114605 [Buttiauxella sp. 3AFRM03]
MVSRFVPDSGDVIWIDFDPVAGHEQGGNRPAVVLSPFAYNNKVGLLLCVPCTTKAKDYPFEVELSGERESVALADQITCV
DWRARKVTKKGSVSVAELADIRAKAKTLIG

Nucleotide

• Length: 333 bp

>T113048 NZ_CP033076:114273-114605 [Buttiauxella sp. 3AFRM03]
ATGGTTTCACGTTTTGTACCGGATTCTGGCGATGTTATCTGGATTGATTTTGACCCTGTAGCAGGACACGAACAGGGCGG
TAATCGCCCTGCCGTGGTGCTGAGCCCATTTGCATATAATAATAAAGTGGGTTTACTACTCTGCGTTCCCTGTACAACCA
AAGCAAAAGACTATCCGTTTGAGGTTGAACTCTCTGGCGAACGTGAAAGCGTAGCCCTGGCCGATCAAATCACATGCGTT
GATTGGCGAGCCAGAAAGGTGACAAAAAAGGGAAGCGTTAGTGTTGCCGAACTGGCTGATATAAGGGCTAAAGCAAAAAC
ACTCATTGGATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8713.15 Da
• Isoelectric Point: 4.9849

>AT113048 WP_064547184.1 NZ_CP033076:114028-114273 [Buttiauxella sp. 3AFRM03]
MSKVIVKKWGNSPSVRLPVAIMQAASLNVDDTVDISVEDGRIVIVPVKTKEYSLDALLAGITPDNIHEKVDFGAPVGKEL
I

Nucleotide

• Length: 246 bp

>AT113048 NZ_CP033076:114028-114273 [Buttiauxella sp. 3AFRM03]
ATGTCGAAGGTAATAGTGAAAAAATGGGGTAACAGCCCATCCGTACGGCTTCCTGTGGCCATTATGCAAGCGGCATCATT
GAATGTGGATGATACCGTTGATATTTCGGTTGAAGACGGCCGTATTGTCATTGTACCGGTGAAAACGAAGGAATATTCCC
TTGATGCGCTGTTAGCCGGGATTACTCCTGACAACATCCATGAAAAAGTTGATTTTGGCGCTCCGGTCGGTAAGGAGCTG
ATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G2I9E0