Detailed information of TA system TA113047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 8194..8812
• Replicon: plasmid pBTX_57
• Accession: NZ_CP033075
• Organism: Buttiauxella sp. 3AFRM03

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D8682_RS00870
• Protein ID: WP_121813209.1
• Coordinates: 8194..8373 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D8682_RS00875
• Protein ID: WP_121813210.1
• Coordinates: 8402..8812 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8682_RS26585	3395..3565	-	171	WP_162302955.1	hypothetical protein	-
D8682_RS00850	3653..4363	-	711	WP_121813206.1	hypothetical protein	-
D8682_RS00855	5059..5829	+	771	WP_121813207.1	site-specific integrase	-
D8682_RS00860	6977..7594	+	618	WP_071700969.1	ParA family protein	-
D8682_RS00865	7647..7883	+	237	WP_121813208.1	DNA partition complex ParG	-
D8682_RS00870	8194..8373	+	180	WP_121813209.1	type II toxin-antitoxin system HicA family toxin	Toxin
D8682_RS00875	8402..8812	+	411	WP_121813210.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D8682_RS00880	9023..9505	-	483	WP_121813211.1	hypothetical protein	-
D8682_RS00890	9793..12045	+	2253	WP_202914343.1	M23 family metallopeptidase	-
D8682_RS00895	12045..12695	+	651	WP_121813213.1	hypothetical protein	-
D8682_RS26590	12804..13070	+	267	WP_162302956.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57691	57691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6729.90 Da
• Isoelectric Point: 11.1475

>T113047 WP_121813209.1 NZ_CP033075:8194-8373 [Buttiauxella sp. 3AFRM03]
MDSRRLIAEIKEDGWILVRVNGSHHHFTHPTKPGLVTIQHPKKDLPIKTVKSIRKQAGI

Nucleotide

• Length: 180 bp

>T113047 NZ_CP033075:8194-8373 [Buttiauxella sp. 3AFRM03]
ATGGATAGCAGAAGGTTAATTGCAGAAATCAAGGAAGATGGTTGGATACTGGTAAGAGTAAATGGAAGTCATCATCACTT
TACTCACCCGACAAAACCGGGACTGGTAACCATACAGCACCCGAAAAAGGACTTACCCATTAAAACGGTTAAGAGCATCA
GGAAACAAGCTGGGATTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14468.51 Da
• Isoelectric Point: 4.3945

>AT113047 WP_121813210.1 NZ_CP033075:8402-8812 [Buttiauxella sp. 3AFRM03]
MLYPIAIEAGDEQHAYGVTVPDLPGCFSAGDTMEEAIANAKEAITGHIELLVEMGGDIPAVSSVGALAKDPEFAGYTWAV
VDIDVTRLLGGSEKINVTLPKSLIDRIDRCVATHPEFKTRSGFLAQVALERIARTA

Nucleotide

• Length: 411 bp

>AT113047 NZ_CP033075:8402-8812 [Buttiauxella sp. 3AFRM03]
ATGTTATATCCAATTGCGATTGAAGCAGGCGACGAACAACATGCATATGGTGTGACAGTGCCTGATTTACCTGGTTGTTT
CTCAGCTGGGGATACGATGGAAGAAGCGATTGCTAATGCCAAAGAAGCGATCACTGGTCATATTGAATTGCTGGTTGAAA
TGGGAGGGGATATTCCAGCCGTTTCATCAGTTGGTGCGCTGGCCAAAGATCCAGAATTTGCCGGTTATACCTGGGCTGTC
GTTGATATTGACGTAACGCGCTTACTTGGCGGCTCAGAAAAAATTAACGTAACACTGCCTAAATCTCTAATTGACCGGAT
TGATCGCTGTGTAGCAACACATCCCGAGTTTAAAACCCGCTCGGGCTTTCTTGCTCAAGTCGCGTTAGAAAGGATTGCAA
GAACAGCTTAA