Detailed information of TA system TA113000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 66266..66888
• Replicon: chromosome
• Accession: NZ_CP033055
• Organism: Serratia sp. 3ACOL1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: U2M374
• Locus tag: D9980_RS00325
• Protein ID: WP_021180142.1
• Coordinates: 66266..66469 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: U2NAN4
• Locus tag: D9980_RS00330
• Protein ID: WP_021180141.1
• Coordinates: 66520..66888 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9980_RS00300	62078..62566	+	489	WP_121606054.1	YbaY family lipoprotein	-
D9980_RS00305	62618..62932	-	315	WP_024484391.1	MGMT family protein	-
D9980_RS00315	63334..65121	-	1788	WP_121606055.1	adenine deaminase	-
D9980_RS00320	65258..66184	+	927	WP_024484393.1	LysR family transcriptional regulator	-
D9980_RS00325	66266..66469	-	204	WP_021180142.1	hemolysin expression modulator Hha	Toxin
D9980_RS00330	66520..66888	-	369	WP_021180141.1	Hha toxicity modulator TomB	Antitoxin
D9980_RS00335	67046..67399	-	354	WP_121608919.1	hypothetical protein	-
D9980_RS00340	67779..68471	+	693	WP_121606056.1	ABC transporter ATP-binding protein	-
D9980_RS00345	68471..69328	+	858	WP_121606057.1	metal ABC transporter permease	-
D9980_RS00350	69354..70232	+	879	WP_121606058.1	metal ABC transporter substrate-binding protein	-
D9980_RS00355	70342..70482	-	141	WP_071784952.1	type B 50S ribosomal protein L36	-
D9980_RS00360	70495..70752	-	258	WP_024484398.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8099.48 Da
• Isoelectric Point: 6.9770

>T113000 WP_021180142.1 NZ_CP033055:c66469-66266 [Serratia sp. 3ACOL1]
MTKIDYLMRLRKCTTIETLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 204 bp

>T113000 NZ_CP033055:c66469-66266 [Serratia sp. 3ACOL1]
ATGACAAAAATTGACTATCTGATGCGTTTGCGTAAATGCACCACGATTGAAACCCTTGAGCGAGTGATCGAGAAAAATAA
GTACGAACTCTCTGACGATGAGCTAGAGCTGTTCTACTCTGCCGCCGATCATCGCCTGGCCGAACTGACCATGAACAAGC
TGTACGATAAAATTCCGGTCGCCGTTTGGAAATTTGTAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14097.78 Da
• Isoelectric Point: 4.5140

>AT113000 WP_021180141.1 NZ_CP033055:c66888-66520 [Serratia sp. 3ACOL1]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYMDEGDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARTFRLFSGEGVCTTMKT

Nucleotide

• Length: 369 bp

>AT113000 NZ_CP033055:c66888-66520 [Serratia sp. 3ACOL1]
ATGGATGAGTATTCGCCTAAAAGGCACGATATTGCTCAACTCAAATTCTTATGTGAAAATTTGTACGACGAAGGCATCGC
TACCTTGGGTGACAGCCACCACGGCTGGGTTAACGATCCAACGTCCGCCGTCAATCTGCAGCTTAATGAGCTGATCGAAC
ATATTGCTTCGTTTGTAATGAGTTTTAAAATTAAATACATGGACGAAGGTGATTTATCGGAACTGGTCGAAGAGTACCTC
GACGATACCTATACCCTGTTTAGCAGCTACGGTATTAATGACTCCGATTTGCGTCGCTGGCAAAAAACCAAGGCACGCAC
ATTCAGATTGTTCTCAGGAGAGGGCGTCTGTACCACGATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: U2M374

Antitoxin

• AlphaFold DB: U2NAN4