Detailed information of TA system TA112998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 728287..728924
• Replicon: chromosome
• Accession: NZ_CP033054
• Organism: Bacillus velezensis strain Bac57

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: D9777_RS03735
• Protein ID: WP_003156187.1
• Coordinates: 728574..728924 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: D9777_RS03730
• Protein ID: WP_003156188.1
• Coordinates: 728287..728568 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9777_RS03710	724652..725251	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
D9777_RS03715	725344..725709	+	366	WP_014304402.1	holo-ACP synthase	-
D9777_RS03720	725874..726881	+	1008	WP_021495080.1	outer membrane lipoprotein carrier protein LolA	-
D9777_RS03725	726998..728167	+	1170	WP_021495079.1	alanine racemase	-
D9777_RS03730	728287..728568	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
D9777_RS03735	728574..728924	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
D9777_RS03740	729042..729863	+	822	WP_003156182.1	STAS domain-containing protein	-
D9777_RS03745	729868..730233	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
D9777_RS03750	730236..730637	+	402	WP_003156178.1	anti-sigma regulatory factor	-
D9777_RS03755	730649..731656	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
D9777_RS03760	731720..732049	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
D9777_RS03765	732046..732528	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
D9777_RS03770	732494..733282	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
D9777_RS03775	733282..733884	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T112998 WP_003156187.1 NZ_CP033054:728574-728924 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T112998 NZ_CP033054:728574-728924 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT112998 WP_003156188.1 NZ_CP033054:728287-728568 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT112998 NZ_CP033054:728287-728568 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5