Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 674736..675372 Replicon chromosome
Accession NZ_CP033052
Organism Bacillus vallismortis strain Bac111

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag D9779_RS03500 Protein ID WP_003156187.1
Coordinates 675022..675372 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag D9779_RS03495 Protein ID WP_003225183.1
Coordinates 674736..675017 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9779_RS03475 671094..671693 - 600 WP_121641936.1 rhomboid family intramembrane serine protease -
D9779_RS03480 671788..672153 + 366 WP_010331253.1 holo-ACP synthase -
D9779_RS03485 672318..673334 + 1017 WP_082022756.1 outer membrane lipoprotein carrier protein LolA -
D9779_RS03490 673449..674618 + 1170 WP_121641937.1 alanine racemase -
D9779_RS03495 674736..675017 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
D9779_RS03500 675022..675372 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
D9779_RS03505 675488..676312 + 825 WP_121641938.1 RsbT co-antagonist protein RsbRA -
D9779_RS03510 676317..676682 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
D9779_RS03515 676686..677087 + 402 WP_121641939.1 serine/threonine-protein kinase RsbT -
D9779_RS03520 677099..678106 + 1008 WP_039075784.1 phosphoserine phosphatase RsbU -
D9779_RS03525 678168..678497 + 330 WP_010331248.1 anti-sigma factor antagonist RsbV -
D9779_RS03530 678494..678976 + 483 WP_003234299.1 anti-sigma B factor RsbW -
D9779_RS03535 678942..679730 + 789 WP_010331247.1 RNA polymerase sigma factor SigB -
D9779_RS03540 679730..680329 + 600 WP_039075782.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T112996 WP_003156187.1 NZ_CP033052:675022-675372 [Bacillus vallismortis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T112996 NZ_CP033052:675022-675372 [Bacillus vallismortis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT112996 WP_003225183.1 NZ_CP033052:674736-675017 [Bacillus vallismortis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT112996 NZ_CP033052:674736-675017 [Bacillus vallismortis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References