Detailed information of TA system TA112995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 2637444..2638092
• Replicon: chromosome
• Accession: NZ_CP033049
• Organism: Virgibacillus halodenitrificans strain Bac324

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A6L8JWB3
• Locus tag: D9778_RS13520
• Protein ID: WP_019379217.1
• Coordinates: 2637444..2637803 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: D9778_RS13525
• Protein ID: WP_019379218.1
• Coordinates: 2637808..2638092 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9778_RS13485	2632647..2633438	-	792	WP_060681900.1	RNA polymerase sigma factor SigB	-
D9778_RS13490	2633410..2633886	-	477	WP_019379211.1	anti-sigma B factor RsbW	-
D9778_RS13495	2633886..2634218	-	333	WP_060681899.1	STAS domain-containing protein	-
D9778_RS13500	2634325..2635338	-	1014	WP_121615801.1	PP2C family protein-serine/threonine phosphatase	-
D9778_RS13505	2635447..2635848	-	402	WP_121615802.1	anti-sigma regulatory factor	-
D9778_RS13510	2635851..2636207	-	357	WP_019379215.1	STAS domain-containing protein	-
D9778_RS13515	2636213..2637082	-	870	WP_121615803.1	RsbT co-antagonist protein RsbRA	-
D9778_RS13520	2637444..2637803	-	360	WP_019379217.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D9778_RS13525	2637808..2638092	-	285	WP_019379218.1	hypothetical protein	Antitoxin
D9778_RS13530	2638255..2639379	-	1125	WP_019379219.1	alanine racemase	-
D9778_RS13535	2639570..2640580	-	1011	WP_121615804.1	outer membrane lipoprotein carrier protein LolA	-
D9778_RS13540	2640668..2642188	-	1521	WP_121615805.1	NAD(P)H-hydrate dehydratase	-
D9778_RS13545	2642248..2642607	-	360	WP_060681891.1	holo-ACP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13377.53 Da
• Isoelectric Point: 8.4940

>T112995 WP_019379217.1 NZ_CP033049:c2637803-2637444 [Virgibacillus halodenitrificans]
MIVQRGEVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRNSVILLEQI
RTLDKQRLTDKITKLDAEMMNKINQALEISLGLKDMYDH

Nucleotide

• Length: 360 bp

>T112995 NZ_CP033049:c2637803-2637444 [Virgibacillus halodenitrificans]
TTGATCGTTCAAAGAGGTGAAGTATATTTCGCCGACTTATCCCCTGTGGTTGGATCGGAGCAGGGAGGCGTGCGCCCGGT
ATTGATCCTTCAAAATGATATAGGTAATCGGTTTAGCCCGACCGTTATTGTGGCAGCTATTACTGCGCAAATTCAAAAGG
CTAAGTTACCTACTCATGTTGAAATTAATGCAAAACGCTACGGATTCGACCGGAATTCTGTTATTCTGCTGGAACAAATC
CGTACATTGGATAAACAGAGATTAACAGATAAAATAACTAAACTTGATGCTGAAATGATGAATAAAATAAATCAGGCTCT
GGAAATTAGTCTGGGGCTGAAAGATATGTATGACCATTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10910.24 Da
• Isoelectric Point: 4.3735

>AT112995 WP_019379218.1 NZ_CP033049:c2638092-2637808 [Virgibacillus halodenitrificans]
MSESLQEILVKIPKNLLNEVDGLMRYENSDLSDFICQATRSYLQHKKDEHIQQFRETMQKGYEEMGRINLTIASEAFQAE
EEAENTLERSVIGV

Nucleotide

• Length: 285 bp

>AT112995 NZ_CP033049:c2638092-2637808 [Virgibacillus halodenitrificans]
TTGTCAGAGAGCTTACAGGAGATCTTAGTGAAAATACCAAAAAACCTATTGAATGAGGTGGATGGATTAATGAGATATGA
AAATAGTGACTTAAGTGATTTCATTTGTCAGGCTACAAGAAGCTACTTGCAGCATAAAAAAGACGAACATATCCAACAAT
TCCGTGAGACAATGCAAAAAGGCTATGAAGAAATGGGTCGCATTAATCTAACAATTGCTTCGGAAGCTTTTCAAGCTGAA
GAGGAGGCTGAAAACACCTTAGAGCGCTCTGTGATCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A6L8JWB3