Detailed information of TA system TA112993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 3235847..3236495
• Replicon: chromosome
• Accession: NZ_CP033048
• Organism: Virgibacillus sp. Bac330

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A3E0WNY0
• Locus tag: D9780_RS15070
• Protein ID: WP_073013075.1
• Coordinates: 3235847..3236206 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: D9780_RS15075
• Protein ID: WP_077706817.1
• Coordinates: 3236211..3236495 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9780_RS15040	3231584..3232069	-	486	WP_170861786.1	anti-sigma B factor RsbW	-
D9780_RS15045	3232066..3232401	-	336	WP_116278896.1	STAS domain-containing protein	-
D9780_RS15050	3232669..3233682	-	1014	WP_073013083.1	PP2C family protein-serine/threonine phosphatase	-
D9780_RS15055	3233886..3234287	-	402	WP_073013081.1	anti-sigma regulatory factor	-
D9780_RS15060	3234291..3234656	-	366	WP_170861785.1	STAS domain-containing protein	-
D9780_RS15065	3234653..3235522	-	870	WP_121640652.1	RsbT co-antagonist protein RsbRA	-
D9780_RS15070	3235847..3236206	-	360	WP_073013075.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D9780_RS15075	3236211..3236495	-	285	WP_077706817.1	antitoxin	Antitoxin
D9780_RS15080	3236947..3238068	-	1122	WP_073013071.1	alanine racemase	-
D9780_RS15085	3238489..3239499	-	1011	WP_073013069.1	outer membrane lipoprotein carrier protein LolA	-
D9780_RS15090	3239671..3241191	-	1521	WP_121640653.1	NAD(P)H-hydrate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13413.60 Da
• Isoelectric Point: 7.0134

>T112993 WP_073013075.1 NZ_CP033048:c3236206-3235847 [Virgibacillus sp. Bac330]
MIVQRGEVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKQYGFDRDSVILLEQI
RTLDKQRLTDKITKLDKEKMKMINQALEISLGLKDMYDQ

Nucleotide

• Length: 360 bp

>T112993 NZ_CP033048:c3236206-3235847 [Virgibacillus sp. Bac330]
TTGATCGTACAAAGAGGCGAAGTGTATTTCGCTGACCTTTCCCCTGTTGTTGGATCAGAGCAGGGAGGCGTCCGCCCGGT
ATTGATCCTGCAAAATGATATCGGGAATCGTTTCAGTCCTACCGTTATTGTGGCTGCAATTACAGCACAAATTCAAAAAG
CCAAACTCCCTACACATGTGGAGATTGATGCAAAACAATATGGGTTTGACCGTGATTCCGTTATTTTGCTAGAGCAGATA
CGAACACTTGATAAACAAAGGCTAACAGATAAAATTACAAAGTTAGATAAAGAAAAGATGAAAATGATTAATCAAGCATT
AGAAATCAGTTTAGGGCTGAAGGATATGTATGATCAATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10898.32 Da
• Isoelectric Point: 4.5792

>AT112993 WP_077706817.1 NZ_CP033048:c3236495-3236211 [Virgibacillus sp. Bac330]
MSESLQEVLVKIPKNLLNEVDGLMKYENSDLSDFICQATRNYLQHKRDEHFQQFRETMEKGYAEMGRINLTIASEAFQAE
EEAKITLERSVIGV

Nucleotide

• Length: 285 bp

>AT112993 NZ_CP033048:c3236495-3236211 [Virgibacillus sp. Bac330]
TTGTCAGAAAGTTTGCAAGAAGTATTAGTGAAAATACCGAAAAACCTGTTAAATGAGGTGGATGGTCTGATGAAATATGA
GAATAGTGATCTAAGTGATTTCATATGTCAGGCAACTAGAAACTATCTTCAGCATAAAAGAGATGAGCATTTTCAACAAT
TTCGCGAAACCATGGAAAAGGGCTATGCAGAAATGGGTCGCATTAATTTGACAATTGCTTCCGAAGCTTTTCAAGCGGAA
GAAGAAGCAAAAATCACCTTGGAACGCTCTGTGATCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A3E0WNY0