Detailed information of TA system TA112989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4381958..4382594
• Replicon: chromosome
• Accession: NZ_CP033043
• Organism: Metabacillus litoralis strain Bac94

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0M2PN83
• Locus tag: D9842_RS21470
• Protein ID: WP_026562078.1
• Coordinates: 4382244..4382594 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: D9842_RS21465
• Protein ID: WP_098797598.1
• Coordinates: 4381958..4382239 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9842_RS21445	4377683..4378402	-	720	WP_121664239.1	rhomboid family intramembrane serine protease	-
D9842_RS21450	4378536..4378901	+	366	WP_121664240.1	holo-ACP synthase	-
D9842_RS21455	4379117..4380136	+	1020	WP_121664241.1	outer membrane lipoprotein carrier protein LolA	-
D9842_RS21460	4380402..4381547	+	1146	WP_121664242.1	alanine racemase	-
D9842_RS21465	4381958..4382239	+	282	WP_098797598.1	antitoxin endoai	Antitoxin
D9842_RS21470	4382244..4382594	+	351	WP_026562078.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
D9842_RS21475	4382946..4383776	+	831	WP_121664243.1	STAS domain-containing protein	-
D9842_RS21480	4383780..4384142	+	363	WP_098797600.1	STAS domain-containing protein	-
D9842_RS21485	4384146..4384547	+	402	WP_098797601.1	anti-sigma regulatory factor	-
D9842_RS21490	4384558..4385565	+	1008	WP_121664244.1	PP2C family protein-serine/threonine phosphatase	-
D9842_RS21495	4385621..4385953	+	333	WP_121664245.1	anti-sigma factor antagonist	-
D9842_RS21500	4385950..4386432	+	483	WP_121664246.1	anti-sigma B factor RsbW	-
D9842_RS21505	4386398..4387189	+	792	WP_121664247.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13020.06 Da
• Isoelectric Point: 4.8781

>T112989 WP_026562078.1 NZ_CP033043:4382244-4382594 [Metabacillus litoralis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T112989 NZ_CP033043:4382244-4382594 [Metabacillus litoralis]
TTGATTGTTAAACGAGGCGACGTTTATTTTGCCGATCTTTCTCCAGTTGTAGGTTCAGAGCAAGGTGGCGTACGTCCTGT
ATTAATTATTCAAAACGATATTGGGAACCGCTTTAGTCCTACAGTTATTATAGCGGCAATTACGGCCCAAATTCAAAAAG
CAAAATTGCCAACTCATGTTGAGATTGATGCTAAGCGTTACGGGTTTGAAAGGGATTCGGTTATCCTGCTTGAACAGATT
CGAACGATTGATAAACAAAGGCTTACTGATAAAATTACCCATCTTGATGATGAGATGATGGATAAAGTTGATGAAGCCTT
ACAAATAAGCTTAGGACTTATCGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10598.08 Da
• Isoelectric Point: 5.8851

>AT112989 WP_098797598.1 NZ_CP033043:4381958-4382239 [Metabacillus litoralis]
VSESSATTEILIQLPQALVSELDVLVKQENGNRNELIYQATKMYIRERKKRQIRESMRRGYMEMAKINLNIASEAFLAES
EADHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT112989 NZ_CP033043:4381958-4382239 [Metabacillus litoralis]
GTGTCTGAATCCAGCGCAACAACAGAAATTTTAATTCAGTTACCTCAAGCATTAGTATCAGAATTGGATGTATTGGTAAA
ACAAGAGAACGGGAACAGAAACGAGCTTATTTATCAAGCGACAAAAATGTATATTCGTGAGCGTAAAAAGCGCCAAATTC
GTGAGTCTATGAGACGTGGTTACATGGAAATGGCAAAAATCAATTTGAATATTGCATCTGAAGCATTTTTGGCAGAATCT
GAGGCTGACCATACCGTTGAACGCTTAGTCAGCGGGGGTTAA

Structures

Toxin

• AlphaFold DB: A0A0M2PN83