Detailed information of TA system TA112984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 603549..604174
• Replicon: chromosome
• Accession: NZ_CP033034
• Organism: Agrobacterium fabrum strain 12D13

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q7D0B1
• Locus tag: At12D13_RS03105
• Protein ID: WP_006311244.1
• Coordinates: 603815..604174 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q7D0B2
• Locus tag: At12D13_RS03100
• Protein ID: WP_010971270.1
• Coordinates: 603549..603815 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
At12D13_RS03075	598574..599398	+	825	WP_080810000.1	class D beta-lactamase	-
At12D13_RS03080	599438..600046	+	609	WP_111805475.1	class I SAM-dependent methyltransferase	-
At12D13_RS03085	600222..602807	+	2586	WP_162991746.1	heavy metal translocating P-type ATPase	-
At12D13_RS03090	602804..603226	+	423	WP_006311241.1	Cu(I)-responsive transcriptional regulator	-
At12D13_RS03095	603279..603479	+	201	WP_006311242.1	heavy-metal-associated domain-containing protein	-
At12D13_RS03100	603549..603815	+	267	WP_010971270.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
At12D13_RS03105	603815..604174	+	360	WP_006311244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
At12D13_RS26685	604182..604430	-	249	WP_162991747.1	hypothetical protein	-
At12D13_RS03115	604609..605997	+	1389	WP_174047372.1	MFS transporter	-
At12D13_RS03120	606077..606250	-	174	WP_010971273.1	hypothetical protein	-
At12D13_RS03125	606319..608037	-	1719	WP_006311254.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

• SpoVT-AbrB domain (14-52)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12857.04 Da
• Isoelectric Point: 8.2782

>T112984 WP_006311244.1 NZ_CP033034:603815-604174 [Agrobacterium fabrum]
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA

Nucleotide

• Length: 360 bp

>T112984 NZ_CP033034:603815-604174 [Agrobacterium fabrum]
ATGGTCCGCAACCAGATCCCCAAGCGCGGCGACGTTTATCTGGTTGATCTAAACCCTGTCGTAGGCAGCGAAATCAAGGA
CGAACATCGCTGTGTCGTCATCACGCCCAGAGAAATTAACGCGGTCGGACTGTGTCTCGTCGTCCCGGTGACCACCGGCG
GCATGTTTACGCGCAAGGCAGGGCTTGCCGTAAATATATCCGGCCACAAGACAACGGGCGTCGCTTTGTGCAATCAGGTG
AGAAGCATGGATATCGTCGCCCGGGTTGCCCAGAAGAAAGCGAAATATATCGAAACCCTCGATGATGCGACGATCGATGA
AATCGCCGGGCGCGTCATCAGCATGATCGATCCAGCTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9423.02 Da
• Isoelectric Point: 5.1106

>AT112984 WP_010971270.1 NZ_CP033034:603549-603815 [Agrobacterium fabrum]
MTVTTKIRRQGGAAVMTIPPALLKMLGLEIGEQLTLEVDNGALVASPVRLEKKRFTLAELLDGAEEVAALNARERAWDTA
PPVGKEAL

Nucleotide

• Length: 267 bp

>AT112984 NZ_CP033034:603549-603815 [Agrobacterium fabrum]
ATGACCGTGACTACGAAAATAAGACGCCAGGGCGGCGCGGCGGTGATGACGATCCCGCCCGCTCTTTTGAAGATGCTGGG
GCTGGAAATCGGTGAGCAACTGACGCTCGAGGTCGATAATGGCGCTCTGGTGGCAAGCCCGGTTCGACTGGAAAAGAAAC
GCTTCACGCTGGCCGAATTACTGGATGGTGCAGAGGAAGTGGCAGCTCTAAACGCCAGGGAGCGGGCGTGGGACACAGCG
CCTCCTGTCGGCAAAGAAGCGTTGTGA

Structures

Toxin

• AlphaFold DB: Q7D0B1

Antitoxin

• AlphaFold DB: Q7D0B2