Detailed information of TA system TA112982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 345368..345848
• Replicon: chromosome
• Accession: NZ_CP033034
• Organism: Agrobacterium fabrum strain 12D13

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A9CK22
• Locus tag: At12D13_RS01690
• Protein ID: WP_010971059.1
• Coordinates: 345368..345637 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A9CK21
• Locus tag: At12D13_RS01695
• Protein ID: WP_010971060.1
• Coordinates: 345621..345848 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
At12D13_RS01660	340419..341195	-	777	WP_121689978.1	L,D-transpeptidase	-
At12D13_RS01665	341434..342063	+	630	WP_121689979.1	DNA-3-methyladenine glycosylase I	-
At12D13_RS01670	342060..342503	+	444	WP_121689980.1	hypothetical protein	-
At12D13_RS01675	342529..343572	-	1044	WP_035256280.1	YeiH family protein	-
At12D13_RS01680	343654..344571	+	918	WP_010971057.1	LysR family transcriptional regulator	-
At12D13_RS01685	344597..345361	-	765	WP_121689981.1	hypothetical protein	-
At12D13_RS01690	345368..345637	-	270	WP_010971059.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
At12D13_RS01695	345621..345848	-	228	WP_010971060.1	ribbon-helix-helix protein, CopG family	Antitoxin
At12D13_RS01700	346005..347525	+	1521	WP_121689982.1	histidine--tRNA ligase	-
At12D13_RS01710	347727..348854	+	1128	WP_006313159.1	ATP phosphoribosyltransferase regulatory subunit	-
At12D13_RS01715	348851..349543	+	693	WP_121689983.1	ATP phosphoribosyltransferase	-
At12D13_RS01720	349607..350053	-	447	WP_162991725.1	DoxX family protein	-
At12D13_RS01725	350209..350643	-	435	WP_121689985.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10746.46 Da
• Isoelectric Point: 9.9414

>T112982 WP_010971059.1 NZ_CP033034:c345637-345368 [Agrobacterium fabrum]
VIWTIEYHTLVQKEMRKINPEVRRRIRSFLHERLAALDDPRQIGATLQGSELGNFWRYRVGDYRIICDIQDQKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T112982 NZ_CP033034:c345637-345368 [Agrobacterium fabrum]
GTGATCTGGACAATTGAATATCACACGCTTGTGCAGAAAGAGATGCGCAAAATAAATCCGGAAGTGCGCCGGCGCATCCG
CAGTTTTCTCCATGAAAGGCTGGCCGCACTGGACGACCCGCGCCAGATCGGCGCTACACTGCAAGGTTCCGAACTCGGAA
ATTTCTGGCGTTACCGGGTGGGCGATTACCGCATCATCTGCGATATACAGGACCAAAAGCTGGTCGTTCTGGTGGTCGAA
ATCGGCCATCGCCGTGAAATTTACCGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8937.97 Da
• Isoelectric Point: 4.7254

>AT112982 WP_010971060.1 NZ_CP033034:c345848-345621 [Agrobacterium fabrum]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKIISAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT112982 NZ_CP033034:c345848-345621 [Agrobacterium fabrum]
ATGAGCAAACAGACGGCCATCCGCCTGCCGGACGAGACCTATGAGCGGCTGAAGGCGCTGTCTGAGCGGACCGGCCGCAC
ATCCGCCTATTATATCCGCGAGGCGATCGAGAAGCATATCGAGGATATGGAAGACCTCTATCTTGCCGAAGAGGCGACGC
GGCGCATTCAGCGCGGAGAATCGAAGATTATAAGCGCCGAGGAGTTCTGGCGTGATCTGGACAATTGA

Structures

Toxin

• AlphaFold DB: A9CK22

Antitoxin

• AlphaFold DB: A9CK21