Detailed information of TA system TA112978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 88532..89202
• Replicon: plasmid pAt1D132a
• Accession: NZ_CP033024
• Organism: Agrobacterium fabrum strain 1D132

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: At1D132_RS24430
• Protein ID: WP_121652003.1
• Coordinates: 88783..89202 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: At1D132_RS24425
• Protein ID: WP_121652040.1
• Coordinates: 88532..88786 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
At1D132_RS24410	85245..85736	+	492	WP_080814697.1	nitrate reductase cytochrome c-type subunit	-
At1D132_RS24415	85733..86434	+	702	WP_080814696.1	cytochrome c3 family protein	-
At1D132_RS24420	87100..87342	+	243	WP_080814695.1	hypothetical protein	-
At1D132_RS24425	88532..88786	+	255	WP_121652040.1	plasmid stabilization protein	Antitoxin
At1D132_RS24430	88783..89202	+	420	WP_121652003.1	type II toxin-antitoxin system VapC family toxin	Toxin
At1D132_RS24435	89380..90552	-	1173	WP_121652004.1	GGDEF domain-containing protein	-
At1D132_RS24440	91479..92399	-	921	WP_121652005.1	efflux RND transporter periplasmic adaptor subunit	-
At1D132_RS24445	92404..93117	-	714	WP_121652006.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..306515	306515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15090.35 Da
• Isoelectric Point: 5.1239

>T112978 WP_121652003.1 NZ_CP033024:88783-89202 [Agrobacterium fabrum]
MILLDTNVISEPWKPVPDEAVIAWLDAQVLETLFISSITIAELRFGIAAMPFGKRQTTLRDRLEGEVLPHFSQRILSFDL
ATSQFYSDLMAHARVSGKAIGTADGYIAATAAANGLAIATRDTSPFEAAGLKVINPWSK

Nucleotide

• Length: 420 bp

>T112978 NZ_CP033024:88783-89202 [Agrobacterium fabrum]
ATGATCTTGCTGGACACCAACGTTATATCTGAGCCGTGGAAACCGGTTCCTGATGAGGCGGTAATTGCGTGGTTGGACGC
ACAAGTTCTCGAAACGCTTTTCATATCCTCAATAACGATTGCTGAACTTCGCTTCGGGATCGCTGCCATGCCTTTTGGGA
AACGGCAAACTACTCTCCGCGATCGCCTTGAGGGTGAAGTCTTGCCCCATTTTTCCCAGCGCATCCTTTCCTTTGATCTG
GCCACTTCTCAGTTTTATTCAGACCTCATGGCACACGCTCGAGTATCTGGGAAAGCGATTGGCACAGCTGATGGCTACAT
TGCAGCCACAGCGGCCGCAAACGGCCTCGCGATCGCCACGCGCGACACCAGCCCGTTCGAGGCAGCTGGGCTGAAGGTGA
TCAATCCCTGGTCCAAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9331.51 Da
• Isoelectric Point: 6.2300

>AT112978 WP_121652040.1 NZ_CP033024:88532-88786 [Agrobacterium fabrum]
MPAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDIIEAAVRPPERIKLGSLLTAIGRDAELSESDVEALQRSRDKTAAEP
MTFE

Nucleotide

• Length: 255 bp

>AT112978 NZ_CP033024:88532-88786 [Agrobacterium fabrum]
ATGCCGGCAGTCACCATTCGTAATCTTTCGGACGAAACACATCGCGCTCTGCGGGTGCGAGCGGCTCATCATGGCCGGAG
TACCGAGGCCGAAATCCGTGACATTATTGAAGCCGCTGTTCGTCCTCCTGAACGTATCAAGCTCGGATCCTTGCTGACGG
CCATTGGTCGCGACGCCGAACTTTCCGAAAGCGACGTTGAAGCCCTGCAGAGAAGTCGCGACAAGACAGCTGCTGAACCG
ATGACGTTCGAATGA