Detailed information of TA system TA112976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 322550..323036
• Replicon: chromosome
• Accession: NZ_CP033022
• Organism: Agrobacterium fabrum strain 1D132

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: At1D132_RS01590
• Protein ID: WP_092770475.1
• Coordinates: 322550..322819 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A7Z7BLB4
• Locus tag: At1D132_RS01595
• Protein ID: WP_006313156.1
• Coordinates: 322803..323036 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
At1D132_RS01560	317601..318377	-	777	WP_092769784.1	L,D-transpeptidase	-
At1D132_RS01565	318616..319245	+	630	WP_121650353.1	DNA-3-methyladenine glycosylase I	-
At1D132_RS01570	319242..319685	+	444	WP_121650354.1	hypothetical protein	-
At1D132_RS01575	319711..320754	-	1044	WP_121650355.1	YeiH family protein	-
At1D132_RS01580	320836..321753	+	918	WP_010971057.1	LysR family transcriptional regulator	-
At1D132_RS01585	321779..322543	-	765	WP_121650356.1	hypothetical protein	-
At1D132_RS01590	322550..322819	-	270	WP_092770475.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
At1D132_RS01595	322803..323036	-	234	WP_006313156.1	ribbon-helix-helix protein, CopG family	Antitoxin
At1D132_RS01600	323272..324795	+	1524	WP_121650357.1	histidine--tRNA ligase	-
At1D132_RS01605	324800..325168	+	369	WP_010971062.1	VOC family protein	-
At1D132_RS01610	325185..326312	+	1128	WP_121650358.1	ATP phosphoribosyltransferase regulatory subunit	-
At1D132_RS01615	326309..327001	+	693	WP_080810428.1	ATP phosphoribosyltransferase	-
At1D132_RS01620	327067..327513	-	447	WP_006313161.1	DoxX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10447.00 Da
• Isoelectric Point: 10.3398

>T112976 WP_092770475.1 NZ_CP033022:c322819-322550 [Agrobacterium fabrum]
MIWRIEFQRNAVKQLGKLTKSDANRIVSFLTDRVAQDDNPRRAGAALQGSELGNFWRYRVGDYRIICDIQDQKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T112976 NZ_CP033022:c322819-322550 [Agrobacterium fabrum]
TTGATCTGGAGAATTGAATTCCAGCGAAATGCCGTCAAGCAACTGGGCAAGCTGACGAAGAGCGATGCCAATCGCATCGT
TTCATTTCTGACGGACCGCGTTGCCCAGGACGACAATCCGCGCAGAGCCGGCGCAGCGCTGCAAGGTTCCGAGCTCGGAA
ATTTCTGGCGTTACCGGGTGGGCGATTACCGCATCATCTGCGATATACAGGACCAAAAGCTGGTCGTTCTGGTGGTCGAA
ATCGGCCATCGCCGTGAAATTTACCGTTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9223.39 Da
• Isoelectric Point: 4.7543

>AT112976 WP_006313156.1 NZ_CP033022:c323036-322803 [Agrobacterium fabrum]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEAMRKHIANKERTYTLEEVMRDLDLEN

Nucleotide

• Length: 234 bp

>AT112976 NZ_CP033022:c323036-322803 [Agrobacterium fabrum]
ATGAGCAAACAGACGGCCATTCGCCTGCCGGACGAGACCTATGAGCGGCTGAAGGCGCTGTCTGAGCGGACCGGCCGCAC
ATCCGCCTATTACATCCGCGAGGCGATCGAGAAGCATATCGAGGATATGGAAGACCTCTATCTTGCCGAAGAGGCGATGC
GGAAGCACATCGCCAATAAGGAGCGCACCTATACTCTGGAAGAAGTGATGCGTGATCTTGATCTGGAGAATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Z7BLB4