Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4277457..4277954 Replicon chromosome
Accession NZ_CP033020
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag D9Q99_RS20560 Protein ID WP_001173343.1
Coordinates 4277457..4277630 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag D9Q99_RS20565 Protein ID WP_000398619.1
Coordinates 4277658..4277954 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9Q99_RS20550 (4274127) 4274127..4275584 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
D9Q99_RS20555 (4275821) 4275821..4277338 + 1518 WP_001295087.1 lysine--tRNA ligase -
D9Q99_RS20560 (4277457) 4277457..4277630 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
D9Q99_RS20565 (4277658) 4277658..4277954 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
D9Q99_RS20570 (4278181) 4278181..4278453 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
D9Q99_RS20575 (4278465) 4278465..4278695 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
D9Q99_RS20580 (4278876) 4278876..4280507 + 1632 WP_001216477.1 sensor histidine kinase -
D9Q99_RS20585 (4280504) 4280504..4281223 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4261978..4277954 15976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T112975 WP_001173343.1 NZ_CP033020:c4277630-4277457 [Escherichia coli ATCC 8739]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T112975 NZ_CP033020:c4277630-4277457 [Escherichia coli ATCC 8739]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT112975 WP_000398619.1 NZ_CP033020:c4277954-4277658 [Escherichia coli ATCC 8739]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT112975 NZ_CP033020:c4277954-4277658 [Escherichia coli ATCC 8739]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References