Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3755508..3756295 Replicon chromosome
Accession NZ_CP033020
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name yeeV Uniprot ID A0A377E3D6
Locus tag D9Q99_RS18155 Protein ID WP_001194695.1
Coordinates 3755918..3756295 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag D9Q99_RS18150 Protein ID WP_000066236.1
Coordinates 3755508..3755867 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9Q99_RS18105 (3750748) 3750748..3751200 + 453 WP_001020418.1 hypothetical protein -
D9Q99_RS18110 (3751197) 3751197..3751649 + 453 WP_000734138.1 hypothetical protein -
D9Q99_RS18115 (3751712) 3751712..3752254 + 543 WP_001104018.1 DUF4339 domain-containing protein -
D9Q99_RS18120 (3752313) 3752313..3752765 + 453 WP_001061894.1 IrmA family protein -
D9Q99_RS18125 (3752842) 3752842..3753075 + 234 WP_001114682.1 DUF905 domain-containing protein -
D9Q99_RS18130 (3753195) 3753195..3754013 + 819 WP_001234406.1 DUF932 domain-containing protein -
D9Q99_RS18135 (3754281) 3754281..3754751 + 471 WP_000131762.1 antirestriction protein -
D9Q99_RS18140 (3754763) 3754763..3755242 + 480 WP_000437750.1 DNA repair protein RadC -
D9Q99_RS18145 (3755263) 3755263..3755484 + 222 WP_000691981.1 DUF987 domain-containing protein -
D9Q99_RS18150 (3755508) 3755508..3755867 + 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
D9Q99_RS18155 (3755918) 3755918..3756295 + 378 WP_001194695.1 TA system toxin CbtA family protein Toxin
D9Q99_RS18160 (3756292) 3756292..3756783 + 492 WP_000777682.1 DUF5983 family protein -
D9Q99_RS18165 (3756815) 3756815..3757018 + 204 WP_000413747.1 DUF957 domain-containing protein -
D9Q99_RS18170 (3757099) 3757099..3757943 + 845 Protein_3555 DUF4942 domain-containing protein -
D9Q99_RS18180 (3758285) 3758285..3759016 - 732 WP_001300756.1 DNA polymerase III subunit epsilon -
D9Q99_RS18185 (3759081) 3759081..3759548 + 468 WP_000917883.1 ribonuclease HI -
D9Q99_RS18190 (3759545) 3759545..3760267 - 723 WP_001297210.1 class I SAM-dependent methyltransferase -
D9Q99_RS18195 (3760301) 3760301..3761056 + 756 WP_001052717.1 hydroxyacylglutathione hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14180.97 Da        Isoelectric Point: 7.4209

>T112972 WP_001194695.1 NZ_CP033020:3755918-3756295 [Escherichia coli ATCC 8739]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T112972 NZ_CP033020:3755918-3756295 [Escherichia coli ATCC 8739]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT112972 WP_000066236.1 NZ_CP033020:3755508-3755867 [Escherichia coli ATCC 8739]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT112972 NZ_CP033020:3755508-3755867 [Escherichia coli ATCC 8739]
TTGTCGAATAAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGTCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
GGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377E3D6

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References