112970

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3456202..3456820 Replicon chromosome
Accession NZ_CP033020
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag D9Q99_RS16655 Protein ID WP_001291435.1
Coordinates 3456602..3456820 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag D9Q99_RS16650 Protein ID WP_000344800.1
Coordinates 3456202..3456576 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9Q99_RS16640 (3451291) 3451291..3452484 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
D9Q99_RS16645 (3452507) 3452507..3455656 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
D9Q99_RS16650 (3456202) 3456202..3456576 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
D9Q99_RS16655 (3456602) 3456602..3456820 + 219 WP_001291435.1 HHA domain-containing protein Toxin
D9Q99_RS16660 (3456992) 3456992..3457543 + 552 WP_000102564.1 maltose O-acetyltransferase -
D9Q99_RS16665 (3457659) 3457659..3458129 + 471 WP_000136192.1 YlaC family protein -
D9Q99_RS16670 (3458293) 3458293..3459843 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
D9Q99_RS16675 (3459885) 3459885..3460238 - 354 WP_000878140.1 DUF1428 domain-containing protein -
D9Q99_RS16685 (3460617) 3460617..3460928 + 312 WP_000409911.1 MGMT family protein -
D9Q99_RS16690 (3460959) 3460959..3461531 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T112970 WP_001291435.1 NZ_CP033020:3456602-3456820 [Escherichia coli ATCC 8739]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T112970 NZ_CP033020:3456602-3456820 [Escherichia coli ATCC 8739]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT112970 WP_000344800.1 NZ_CP033020:3456202-3456576 [Escherichia coli ATCC 8739]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT112970 NZ_CP033020:3456202-3456576 [Escherichia coli ATCC 8739]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References