Detailed information of TA system TA112969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3064943..3065648
• Replicon: chromosome
• Accession: NZ_CP033020
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: D9Q99_RS14775
• Protein ID: WP_000539521.1
• Coordinates: 3064943..3065329 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D9Q99_RS14780
• Protein ID: WP_001280945.1
• Coordinates: 3065319..3065648 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9Q99_RS14755 (3060947)	3060947..3061573	+	627	WP_001295292.1	glutathione S-transferase GstB	-
D9Q99_RS14760 (3061570)	3061570..3062685	-	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
D9Q99_RS14765 (3062796)	3062796..3063179	-	384	WP_000497137.1	biofilm formation regulator BssR	-
D9Q99_RS14770 (3063392)	3063392..3064717	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
D9Q99_RS14775 (3064943)	3064943..3065329	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9Q99_RS14780 (3065319)	3065319..3065648	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
D9Q99_RS14785 (3065718)	3065718..3067046	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
D9Q99_RS14790 (3067054)	3067054..3069402	-	2349	WP_012304894.1	EAL domain-containing protein	-
D9Q99_RS14795 (3069580)	3069580..3070491	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T112969 WP_000539521.1 NZ_CP033020:3064943-3065329 [Escherichia coli ATCC 8739]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T112969 NZ_CP033020:3064943-3065329 [Escherichia coli ATCC 8739]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT112969 WP_001280945.1 NZ_CP033020:3065319-3065648 [Escherichia coli ATCC 8739]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT112969 NZ_CP033020:3065319-3065648 [Escherichia coli ATCC 8739]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6