Detailed information of TA system TA112968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2441369..2442007
• Replicon: chromosome
• Accession: NZ_CP033020
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: D9Q99_RS11710
• Protein ID: WP_000813794.1
• Coordinates: 2441831..2442007 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D9Q99_RS11705
• Protein ID: WP_001270286.1
• Coordinates: 2441369..2441785 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9Q99_RS11685 (2436521)	2436521..2437462	-	942	WP_001251313.1	ABC transporter permease	-
D9Q99_RS11690 (2437463)	2437463..2438476	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
D9Q99_RS11695 (2438494)	2438494..2439639	-	1146	WP_000047426.1	ABC transporter substrate-binding protein	-
D9Q99_RS11700 (2439884)	2439884..2441290	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
D9Q99_RS11705 (2441369)	2441369..2441785	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
D9Q99_RS11710 (2441831)	2441831..2442007	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
D9Q99_RS11715 (2442229)	2442229..2442459	+	231	WP_000494244.1	YncJ family protein	-
D9Q99_RS11720 (2442551)	2442551..2444512	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
D9Q99_RS11725 (2444585)	2444585..2445121	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
D9Q99_RS11730 (2445213)	2445213..2446388	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T112968 WP_000813794.1 NZ_CP033020:c2442007-2441831 [Escherichia coli ATCC 8739]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T112968 NZ_CP033020:c2442007-2441831 [Escherichia coli ATCC 8739]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT112968 WP_001270286.1 NZ_CP033020:c2441785-2441369 [Escherichia coli ATCC 8739]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT112968 NZ_CP033020:c2441785-2441369 [Escherichia coli ATCC 8739]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB