Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 871626..872280 Replicon chromosome
Accession NZ_CP033020
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag D9Q99_RS04250 Protein ID WP_000244777.1
Coordinates 871873..872280 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag D9Q99_RS04245 Protein ID WP_000354046.1
Coordinates 871626..871892 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9Q99_RS04220 (866795) 866795..867538 + 744 WP_000951964.1 SDR family oxidoreductase -
D9Q99_RS04225 (867595) 867595..869028 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
D9Q99_RS04230 (869073) 869073..869384 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
D9Q99_RS04235 (869548) 869548..870207 + 660 WP_000250274.1 hemolysin III family protein -
D9Q99_RS04240 (870403) 870403..871383 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
D9Q99_RS04245 (871626) 871626..871892 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
D9Q99_RS04250 (871873) 871873..872280 + 408 WP_000244777.1 protein YgfX Toxin
D9Q99_RS04255 (872320) 872320..872841 - 522 WP_001055874.1 flavodoxin FldB -
D9Q99_RS04260 (872953) 872953..873849 + 897 WP_000806628.1 site-specific tyrosine recombinase XerD -
D9Q99_RS04265 (873874) 873874..874584 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D9Q99_RS04270 (874590) 874590..876323 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T112956 WP_000244777.1 NZ_CP033020:871873-872280 [Escherichia coli ATCC 8739]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T112956 NZ_CP033020:871873-872280 [Escherichia coli ATCC 8739]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT112956 WP_000354046.1 NZ_CP033020:871626-871892 [Escherichia coli ATCC 8739]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT112956 NZ_CP033020:871626-871892 [Escherichia coli ATCC 8739]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References