Detailed information of TA system TA112947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 8622..9144
• Replicon: plasmid p2_W5-6
• Accession: NZ_CP032994
• Organism: Escherichia coli strain W5-6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: D9V54_RS24225
• Protein ID: WP_000220560.1
• Coordinates: 8622..8903 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: D9V54_RS24230
• Protein ID: WP_000121743.1
• Coordinates: 8893..9144 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V54_RS24175	4467..5635	-	1169	Protein_5	relaxase/mobilization nuclease domain-containing protein	-
D9V54_RS24180	5638..6183	-	546	WP_015060502.1	hypothetical protein	-
D9V54_RS25290	6178..6252	-	75	Protein_7	DNA distortion polypeptide 3	-
D9V54_RS24190	6510..6782	-	273	WP_000160399.1	hypothetical protein	-
D9V54_RS24195	6795..6944	-	150	WP_000003880.1	hypothetical protein	-
D9V54_RS24200	7014..7193	-	180	WP_000439078.1	hypothetical protein	-
D9V54_RS24205	7236..7565	-	330	WP_000866648.1	hypothetical protein	-
D9V54_RS24210	7658..7873	-	216	WP_001180116.1	hypothetical protein	-
D9V54_RS24215	7863..8108	-	246	WP_106493875.1	hypothetical protein	-
D9V54_RS24220	8153..8476	-	324	WP_001181903.1	hypothetical protein	-
D9V54_RS24225	8622..8903	-	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9V54_RS24230	8893..9144	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
D9V54_RS24245	10378..11214	+	837	WP_001050931.1	replication initiation protein	-
D9V54_RS24250	11254..11700	+	447	WP_001074386.1	hypothetical protein	-
D9V54_RS24255	11768..12190	+	423	WP_031942371.1	DNA distortion polypeptide 3	-
D9V54_RS24260	12250..13218	-	969	WP_031942372.1	IS5 family transposase	-
D9V54_RS24265	13220..13513	-	294	WP_015060510.1	hypothetical protein	-
D9V54_RS24270	13529..13759	-	231	WP_000051064.1	partitioning protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B	mrkF / mrkD / mrkC / mrkB / mrkA	1..44779	44779
	-	flank	IS/Tn	-	-	12250..13173	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T112947 WP_000220560.1 NZ_CP032994:c8903-8622 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T112947 NZ_CP032994:c8903-8622 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT112947 WP_000121743.1 NZ_CP032994:c9144-8893 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT112947 NZ_CP032994:c9144-8893 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301