Detailed information of TA system TA112939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2351138..2351776
• Replicon: chromosome
• Accession: NZ_CP032992
• Organism: Escherichia coli strain W5-6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: D9V54_RS11680
• Protein ID: WP_001447010.1
• Coordinates: 2351600..2351776 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: D9V54_RS11675
• Protein ID: WP_001270286.1
• Coordinates: 2351138..2351554 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V54_RS11655	2346290..2347231	-	942	WP_001251304.1	ABC transporter permease	-
D9V54_RS11660	2347232..2348245	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
D9V54_RS11665	2348263..2349408	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
D9V54_RS11670	2349653..2351059	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
D9V54_RS11675	2351138..2351554	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
D9V54_RS11680	2351600..2351776	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
D9V54_RS11685	2351998..2352228	+	231	WP_000494244.1	YncJ family protein	-
D9V54_RS11690	2352320..2354281	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
D9V54_RS11695	2354354..2354890	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
D9V54_RS11700	2354943..2356157	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sodB / espR1 / espR1	2109634..2421548	311914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T112939 WP_001447010.1 NZ_CP032992:c2351776-2351600 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T112939 NZ_CP032992:c2351776-2351600 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT112939 WP_001270286.1 NZ_CP032992:c2351554-2351138 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT112939 NZ_CP032992:c2351554-2351138 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8