Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2213552..2214078 Replicon chromosome
Accession NZ_CP032992
Organism Escherichia coli strain W5-6

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag D9V54_RS11010 Protein ID WP_000323025.1
Coordinates 2213791..2214078 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag D9V54_RS11005 Protein ID WP_000534858.1
Coordinates 2213552..2213791 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V54_RS10970 2209280..2209513 - 234 WP_001341330.1 IS66 family insertion sequence element accessory protein TnpB -
D9V54_RS10975 2209561..2209659 - 99 Protein_2087 IS66 family insertion sequence element accessory protein TnpB -
D9V54_RS25220 2209881..2210168 + 288 Protein_2088 hypothetical protein -
D9V54_RS10990 2210937..2212250 + 1314 WP_000589005.1 ISNCY family transposase -
D9V54_RS10995 2212687..2213019 - 333 WP_001301033.1 protein FlxA -
D9V54_RS11000 2213222..2213527 - 306 WP_001326990.1 hypothetical protein -
D9V54_RS11005 2213552..2213791 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
D9V54_RS11010 2213791..2214078 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
D9V54_RS11015 2214150..2214305 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
D9V54_RS11020 2214522..2214773 + 252 WP_000980994.1 hypothetical protein -
D9V54_RS11025 2214840..2215118 + 279 WP_012304870.1 hypothetical protein -
D9V54_RS11030 2215120..2216169 + 1050 WP_001265199.1 DUF968 domain-containing protein -
D9V54_RS11035 2216183..2216935 + 753 WP_001047135.1 antitermination protein -
D9V54_RS24935 2217213..2217302 - 90 WP_120795389.1 hypothetical protein -
D9V54_RS11040 2217357..2217569 - 213 WP_000087756.1 cold shock-like protein CspF -
D9V54_RS11045 2217870..2218085 + 216 WP_000066484.1 cold shock-like protein CspB -
D9V54_RS25360 2218449..2218619 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
D9V54_RS11050 2218839..2219054 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - sodB / espR1 / espR1 2109634..2421548 311914


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T112938 WP_000323025.1 NZ_CP032992:2213791-2214078 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T112938 NZ_CP032992:2213791-2214078 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT112938 WP_000534858.1 NZ_CP032992:2213552-2213791 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT112938 NZ_CP032992:2213552-2213791 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References