Detailed information of TA system TA112927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 958784..959367
• Replicon: chromosome
• Accession: NZ_CP032992
• Organism: Escherichia coli strain W5-6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D9V54_RS04745
• Protein ID: WP_049253269.1
• Coordinates: 959032..959367 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: D9V54_RS04740
• Protein ID: WP_000581941.1
• Coordinates: 958784..959032 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V54_RS04730	955123..956424	+	1302	WP_023281448.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
D9V54_RS04735	956472..958706	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
D9V54_RS04740	958784..959032	+	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
D9V54_RS04745	959032..959367	+	336	WP_049253269.1	endoribonuclease MazF	Toxin
D9V54_RS04750	959438..960229	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
D9V54_RS04755	960457..962094	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
D9V54_RS04760	962182..963480	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12197.18 Da
• Isoelectric Point: 8.7218

>T112927 WP_049253269.1 NZ_CP032992:959032-959367 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQARHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T112927 NZ_CP032992:959032-959367 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTCG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT112927 WP_000581941.1 NZ_CP032992:958784-959032 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT112927 NZ_CP032992:958784-959032 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9