Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 821324..821978 Replicon chromosome
Accession NZ_CP032992
Organism Escherichia coli strain W5-6

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag D9V54_RS04105 Protein ID WP_000244777.1
Coordinates 821571..821978 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag D9V54_RS04100 Protein ID WP_000354046.1
Coordinates 821324..821590 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V54_RS04075 816493..817236 + 744 WP_000951962.1 SDR family oxidoreductase -
D9V54_RS04080 817293..818726 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
D9V54_RS04085 818771..819082 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
D9V54_RS04090 819246..819905 + 660 WP_000250274.1 hemolysin III family protein -
D9V54_RS04095 820101..821081 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
D9V54_RS04100 821324..821590 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
D9V54_RS04105 821571..821978 + 408 WP_000244777.1 toxin CptA Toxin
D9V54_RS04110 822018..822539 - 522 WP_001055874.1 flavodoxin FldB -
D9V54_RS04115 822651..823547 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
D9V54_RS04120 823572..824283 + 712 Protein_790 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D9V54_RS04125 824289..826022 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T112926 WP_000244777.1 NZ_CP032992:821571-821978 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T112926 NZ_CP032992:821571-821978 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT112926 WP_000354046.1 NZ_CP032992:821324-821590 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT112926 NZ_CP032992:821324-821590 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References