Detailed information of TA system TA112913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 48227..48749
• Replicon: plasmid pMCR_W2-5
• Accession: NZ_CP032990
• Organism: Escherichia coli strain W2-5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: D9V55_RS24585
• Protein ID: WP_000638823.1
• Coordinates: 48468..48749 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: D9V55_RS24580
• Protein ID: WP_000121741.1
• Coordinates: 48227..48478 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V55_RS24535	43257..43703	+	447	WP_001243165.1	hypothetical protein	-
D9V55_RS24540	43736..43993	+	258	WP_001542015.1	hypothetical protein	-
D9V55_RS24545	43974..44276	+	303	WP_001360345.1	hypothetical protein	-
D9V55_RS24550	44273..44530	+	258	WP_000739144.1	hypothetical protein	-
D9V55_RS24555	44534..45529	+	996	WP_065304446.1	type IV secretion system protein	-
D9V55_RS24560	45535..46179	+	645	WP_001310442.1	conjugal transfer protein TrbJ	-
D9V55_RS24565	46188..46412	+	225	WP_000713562.1	EexN family lipoprotein	-
D9V55_RS24570	46635..47444	-	810	WP_171004827.1	DNA primase	-
D9V55_RS24575	47492..48127	+	636	WP_015059536.1	hypothetical protein	-
D9V55_RS24580	48227..48478	+	252	WP_000121741.1	hypothetical protein	Antitoxin
D9V55_RS24585	48468..48749	+	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9V55_RS24590	48817..49116	+	300	WP_000835764.1	IncN plasmid KikA protein	-
D9V55_RS24595	49119..50354	+	1236	WP_072652802.1	TcpQ domain-containing protein	-
D9V55_RS24600	50360..50797	+	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
D9V55_RS24605	50916..51314	+	399	WP_001153665.1	hypothetical protein	-
D9V55_RS24610	51335..51919	+	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
D9V55_RS24615	52021..52209	+	189	WP_050567133.1	hypothetical protein	-
D9V55_RS24620	52280..52600	+	321	WP_000362080.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..66380	66380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T112913 WP_000638823.1 NZ_CP032990:48468-48749 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T112913 NZ_CP032990:48468-48749 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT112913 WP_000121741.1 NZ_CP032990:48227-48478 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT112913 NZ_CP032990:48227-48478 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1