112912

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 29949..30442 Replicon plasmid pMCR_W2-5
Accession NZ_CP032990
Organism Escherichia coli strain W2-5

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag D9V55_RS24435 Protein ID WP_001230709.1
Coordinates 30188..30442 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag D9V55_RS24430 Protein ID WP_000151456.1
Coordinates 29949..30191 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V55_RS24390 25241..25540 - 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
D9V55_RS24395 26042..26224 + 183 WP_001428470.1 hypothetical protein -
D9V55_RS24400 26312..26659 - 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
D9V55_RS24405 26656..26904 - 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
D9V55_RS24410 27412..27600 + 189 WP_000957857.1 hypothetical protein -
D9V55_RS24415 27610..28809 + 1200 WP_000948429.1 IS91 family transposase -
D9V55_RS24420 29085..29378 - 294 WP_000673428.1 hypothetical protein -
D9V55_RS24430 29949..30191 - 243 WP_000151456.1 hypothetical protein Antitoxin
D9V55_RS24435 30188..30442 - 255 WP_001230709.1 hypothetical protein Toxin
D9V55_RS24440 30439..30981 - 543 WP_001083904.1 hypothetical protein -
D9V55_RS24445 31083..31598 - 516 WP_001025393.1 J domain-containing protein -
D9V55_RS24450 32002..32331 - 330 WP_000855119.1 hypothetical protein -
D9V55_RS24460 32920..33586 + 667 Protein_37 P-loop NTPase -
D9V55_RS25700 33660..33944 + 285 WP_001162371.1 hypothetical protein -
D9V55_RS24470 33962..34558 - 597 WP_000125165.1 hypothetical protein -
D9V55_RS24475 34708..34869 - 162 WP_000775236.1 type I toxin-antitoxin system Hok family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..66380 66380
- flank IS/Tn - - 27610..28809 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T112912 WP_001230709.1 NZ_CP032990:c30442-30188 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T112912 NZ_CP032990:c30442-30188 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT112912 WP_000151456.1 NZ_CP032990:c30191-29949 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT112912 NZ_CP032990:c30191-29949 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References