Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | hicAB/- |
Location | 26312..26904 | Replicon | plasmid pMCR_W2-5 |
Accession | NZ_CP032990 | ||
Organism | Escherichia coli strain W2-5 |
Toxin (Protein)
Gene name | hicA | Uniprot ID | A0A1I9ZJC5 |
Locus tag | D9V55_RS24405 | Protein ID | WP_000513532.1 |
Coordinates | 26656..26904 (-) | Length | 83 a.a. |
Antitoxin (Protein)
Gene name | hicB | Uniprot ID | A0A1I9ZJ49 |
Locus tag | D9V55_RS24400 | Protein ID | WP_000681609.1 |
Coordinates | 26312..26659 (-) | Length | 116 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
D9V55_RS24360 | 21604..21945 | - | 342 | WP_000730722.1 | plasmid mobilization relaxosome protein MobC | - |
D9V55_RS24365 | 22313..22714 | + | 402 | WP_063084542.1 | hypothetical protein | - |
D9V55_RS24370 | 22732..23484 | + | 753 | WP_000797967.1 | conjugal transfer protein TraL | - |
D9V55_RS24375 | 23486..24193 | + | 708 | WP_063084541.1 | hypothetical protein | - |
D9V55_RS24380 | 24213..24653 | + | 441 | WP_121636733.1 | hypothetical protein | - |
D9V55_RS24385 | 24655..24981 | + | 327 | WP_000206697.1 | hypothetical protein | - |
D9V55_RS24390 | 25241..25540 | - | 300 | WP_034169410.1 | type II toxin-antitoxin system HicB family antitoxin | - |
D9V55_RS24395 | 26042..26224 | + | 183 | WP_001428470.1 | hypothetical protein | - |
D9V55_RS24400 | 26312..26659 | - | 348 | WP_000681609.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
D9V55_RS24405 | 26656..26904 | - | 249 | WP_000513532.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
D9V55_RS24410 | 27412..27600 | + | 189 | WP_000957857.1 | hypothetical protein | - |
D9V55_RS24415 | 27610..28809 | + | 1200 | WP_000948429.1 | IS91 family transposase | - |
D9V55_RS24420 | 29085..29378 | - | 294 | WP_000673428.1 | hypothetical protein | - |
D9V55_RS24430 | 29949..30191 | - | 243 | WP_000151456.1 | hypothetical protein | - |
D9V55_RS24435 | 30188..30442 | - | 255 | WP_001230709.1 | hypothetical protein | - |
D9V55_RS24440 | 30439..30981 | - | 543 | WP_001083904.1 | hypothetical protein | - |
D9V55_RS24445 | 31083..31598 | - | 516 | WP_001025393.1 | J domain-containing protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Mobilizable plasmid | mcr-1.1 | - | 1..66380 | 66380 | |
- | flank | IS/Tn | - | - | 27610..28809 | 1199 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 83 a.a. Molecular weight: 9378.03 Da Isoelectric Point: 10.4800
>T112911 WP_000513532.1 NZ_CP032990:c26904-26656 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
Download Length: 249 bp
>T112911 NZ_CP032990:c26904-26656 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA
Antitoxin
Download Length: 116 a.a. Molecular weight: 13206.97 Da Isoelectric Point: 4.8313
>AT112911 WP_000681609.1 NZ_CP032990:c26659-26312 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
Download Length: 348 bp
>AT112911 NZ_CP032990:c26659-26312 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A1I9ZJC5 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A1I9ZJ49 |