Detailed information of TA system TA112911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 26312..26904
• Replicon: plasmid pMCR_W2-5
• Accession: NZ_CP032990
• Organism: Escherichia coli strain W2-5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: D9V55_RS24405
• Protein ID: WP_000513532.1
• Coordinates: 26656..26904 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: D9V55_RS24400
• Protein ID: WP_000681609.1
• Coordinates: 26312..26659 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V55_RS24360	21604..21945	-	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-
D9V55_RS24365	22313..22714	+	402	WP_063084542.1	hypothetical protein	-
D9V55_RS24370	22732..23484	+	753	WP_000797967.1	conjugal transfer protein TraL	-
D9V55_RS24375	23486..24193	+	708	WP_063084541.1	hypothetical protein	-
D9V55_RS24380	24213..24653	+	441	WP_121636733.1	hypothetical protein	-
D9V55_RS24385	24655..24981	+	327	WP_000206697.1	hypothetical protein	-
D9V55_RS24390	25241..25540	-	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
D9V55_RS24395	26042..26224	+	183	WP_001428470.1	hypothetical protein	-
D9V55_RS24400	26312..26659	-	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D9V55_RS24405	26656..26904	-	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
D9V55_RS24410	27412..27600	+	189	WP_000957857.1	hypothetical protein	-
D9V55_RS24415	27610..28809	+	1200	WP_000948429.1	IS91 family transposase	-
D9V55_RS24420	29085..29378	-	294	WP_000673428.1	hypothetical protein	-
D9V55_RS24430	29949..30191	-	243	WP_000151456.1	hypothetical protein	-
D9V55_RS24435	30188..30442	-	255	WP_001230709.1	hypothetical protein	-
D9V55_RS24440	30439..30981	-	543	WP_001083904.1	hypothetical protein	-
D9V55_RS24445	31083..31598	-	516	WP_001025393.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..66380	66380
	-	flank	IS/Tn	-	-	27610..28809	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T112911 WP_000513532.1 NZ_CP032990:c26904-26656 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T112911 NZ_CP032990:c26904-26656 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT112911 WP_000681609.1 NZ_CP032990:c26659-26312 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT112911 NZ_CP032990:c26659-26312 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49