Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/-
Location 26312..26904 Replicon plasmid pMCR_W2-5
Accession NZ_CP032990
Organism Escherichia coli strain W2-5

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I9ZJC5
Locus tag D9V55_RS24405 Protein ID WP_000513532.1
Coordinates 26656..26904 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1I9ZJ49
Locus tag D9V55_RS24400 Protein ID WP_000681609.1
Coordinates 26312..26659 (-) Length 116 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V55_RS24360 21604..21945 - 342 WP_000730722.1 plasmid mobilization relaxosome protein MobC -
D9V55_RS24365 22313..22714 + 402 WP_063084542.1 hypothetical protein -
D9V55_RS24370 22732..23484 + 753 WP_000797967.1 conjugal transfer protein TraL -
D9V55_RS24375 23486..24193 + 708 WP_063084541.1 hypothetical protein -
D9V55_RS24380 24213..24653 + 441 WP_121636733.1 hypothetical protein -
D9V55_RS24385 24655..24981 + 327 WP_000206697.1 hypothetical protein -
D9V55_RS24390 25241..25540 - 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
D9V55_RS24395 26042..26224 + 183 WP_001428470.1 hypothetical protein -
D9V55_RS24400 26312..26659 - 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
D9V55_RS24405 26656..26904 - 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin Toxin
D9V55_RS24410 27412..27600 + 189 WP_000957857.1 hypothetical protein -
D9V55_RS24415 27610..28809 + 1200 WP_000948429.1 IS91 family transposase -
D9V55_RS24420 29085..29378 - 294 WP_000673428.1 hypothetical protein -
D9V55_RS24430 29949..30191 - 243 WP_000151456.1 hypothetical protein -
D9V55_RS24435 30188..30442 - 255 WP_001230709.1 hypothetical protein -
D9V55_RS24440 30439..30981 - 543 WP_001083904.1 hypothetical protein -
D9V55_RS24445 31083..31598 - 516 WP_001025393.1 J domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..66380 66380
- flank IS/Tn - - 27610..28809 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(20-81)

Antitoxin

(54-104)


Sequences


Toxin        


Download         Length: 83 a.a.        Molecular weight: 9378.03 Da        Isoelectric Point: 10.4800

>T112911 WP_000513532.1 NZ_CP032990:c26904-26656 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T112911 NZ_CP032990:c26904-26656 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13206.97 Da        Isoelectric Point: 4.8313

>AT112911 WP_000681609.1 NZ_CP032990:c26659-26312 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Download         Length: 348 bp

>AT112911 NZ_CP032990:c26659-26312 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1I9ZJC5


Antitoxin

Source ID Structure
AlphaFold DB A0A1I9ZJ49

References