Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 866405..867203 | Replicon | chromosome |
| Accession | NZ_CP032989 | ||
| Organism | Escherichia coli strain W2-5 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | U9XMP3 |
| Locus tag | D9V55_RS04455 | Protein ID | WP_000854735.1 |
| Coordinates | 866405..866782 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A1M1EW42 |
| Locus tag | D9V55_RS04460 | Protein ID | WP_032153712.1 |
| Coordinates | 866829..867203 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| D9V55_RS04420 | 861546..861788 | - | 243 | Protein_829 | IS110 family transposase | - |
| D9V55_RS04425 | 862370..863635 | - | 1266 | WP_032171996.1 | integrase arm-type DNA-binding domain-containing protein | - |
| D9V55_RS04430 | 864099..864425 | - | 327 | WP_000779483.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| D9V55_RS04435 | 864422..864685 | - | 264 | WP_025492088.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| D9V55_RS04440 | 864757..865623 | - | 867 | WP_032153714.1 | DUF4942 domain-containing protein | - |
| D9V55_RS04445 | 865708..865905 | - | 198 | WP_000839265.1 | DUF957 domain-containing protein | - |
| D9V55_RS04450 | 865917..866408 | - | 492 | WP_023155722.1 | hypothetical protein | - |
| D9V55_RS04455 | 866405..866782 | - | 378 | WP_000854735.1 | TA system toxin CbtA family protein | Toxin |
| D9V55_RS04460 | 866829..867203 | - | 375 | WP_032153712.1 | type IV toxin-antitoxin system toxin CbtA | Antitoxin |
| D9V55_RS04465 | 867283..867504 | - | 222 | WP_000692345.1 | DUF987 domain-containing protein | - |
| D9V55_RS04470 | 867573..868049 | - | 477 | WP_001186715.1 | RadC family protein | - |
| D9V55_RS04480 | 868065..868550 | - | 486 | WP_032153711.1 | antirestriction protein | - |
| D9V55_RS04485 | 868642..869460 | - | 819 | WP_001234693.1 | DUF945 domain-containing protein | - |
| D9V55_RS04490 | 869551..869760 | - | 210 | WP_023155727.1 | DUF905 family protein | - |
| D9V55_RS04495 | 869790..870467 | - | 678 | WP_023155728.1 | hypothetical protein | - |
| D9V55_RS04500 | 870586..871470 | - | 885 | WP_024166630.1 | 50S ribosome-binding GTPase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR | papI / papB | 858385..931580 | 73195 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14061.07 Da Isoelectric Point: 8.2904
>T112890 WP_000854735.1 NZ_CP032989:c866782-866405 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ
Download Length: 378 bp
>T112890 NZ_CP032989:c866782-866405 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13697.51 Da Isoelectric Point: 6.6240
>AT112890 WP_032153712.1 NZ_CP032989:c867203-866829 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS
Download Length: 375 bp
>AT112890 NZ_CP032989:c867203-866829 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9XMP3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1M1EW42 |